Intended use:
The test detects cashew DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg cashew / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.
| Art. No. | S3615 |
|---|---|
| Test format | 100 reactions |
| Detection limit | The SureFood® ALLERGEN Cashew PCR has a limit of detection of ≤ 0.4 mg / kg. |
| Instructions | German/English |
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