SureFood® ALLERGEN Mustard
Art. No. S3609
Product info
Intended use
The SureFood® ALLERGEN Mustard is a real-time PCR for the direct, qualitative and / or quantitative detection of specific white mustard (Sinapis alba), indian mustard (Brassica juncea) und black mustard (Brassica nigra) DNA sequences according to directive (EC) 1169/2011.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg mustard / kg food sample and a detection system including a standard series is required.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on AgilentMx3005P, Agilent AriaDx, Applied Biosystems 7500, Bio-Rad CFX96, LTF MyGo Pro, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER, Roche cobas® z 480 Analyzer and Roche LightCycler® 480 II.
Accessories
Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.
| Specifications | |
|---|---|
| Art. No | S3609 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection Limit) | The SureFood® ALLERGEN Mustard PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1. |
| LOQ (Limit of quantification | 1 mg / kg using SureFood® PREP Advanced, protocol 1 |
