Wine analysis
Detection of spoilage organisms in wine
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Wine analysis
Do you want to test wine for beverage spoilers? For fast and specific screening and detection of microbiological spoilage parameters in wine, a wide product line of qPCR kits is offered.
We offer several qualitative real-time PCR test kits for wine analysis, e.g. for detection of the yeast Brettanomyces (Dekkera bruxellensis) which may spoil the taste and flavour of the wine. The following spoilage bacteria and yeasts are of special interest in the wine industry and should be monitored:
Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) is a spoilage yeast in beverages, especially important for the wine industry, where it has been shown to produce high amounts of volatile phenols (4-ethylphenol and 4-ethylguaiacol) imparting off-flavors to red wines. The yeasts of the species Dekkera bruxellensis are responsible for serious economic losses in the wine industry owing to their ability to spoil wines by the production of ethylphenols. Some authorities consider “brett” to be responsible for 90% of the spoilage problems in premium red wines. However, in the production of beer (especially craft beer or Belgian beer specialities), Dekkera bruxellensis is often not considered as contaminant, but as flavor enrichment.
In wine production, the malolactic fermentation is supported by Lactobacillus. During this process, the bacterium Oenococcus oeni can be added as highly specialized bacteria. It effects a transformation of malic acid into milder lactic acid. However, depending on the desired type of wine, the presence of Oenococcus oeni may also be undesirable.
Pediococcus is a genus of gram-positive lactic acid bacteria which are considered as contaminant in wine and beer. However, in some beer styles, the presence of Pediococcus may be desired.
Pichia is a genus of yeasts in the family Saccharomycetaceae. It is used in the fermentation process in winemaking, but it can spoil wine when present in high quantities. Pichia may also cause turbidity and off-flavors in beer.
Saccharomyces is a genus of fungi including various species of yeasts. One of the best known species is Saccharomyces cerevisiae which is used in the production of beer, wine or bread. The species Saccharomyces diastaticus is a spoilage yeast in the wine and beer industry.
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GEN-IAL – DNA Preparation and PCR – Wine
Real-time PCR as a supporting tool for quality assurance throughout the wine-making process.
This video gives a brief overview of the DNA preparation and real-time PCR set-up when analyzing wine or must samples for (spoilage) bacteria and yeast.
Molecularbiological wine analysis
You can find more information on real-time PCR wine analysis in our free brochure.
Test kits for wine analysis
Our GEN-IAL® portfolio includes multiplex screening kits and wine-specific kits. The new GEN-IAL® QuickGEN line is characterized by a simple and rapid sampling and assay process, providing results within two hours after sampling.
| Product | Description | Tube | Art. No. |
|---|---|---|---|
| GEN-IAL® QuickGEN Wine Screening | Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must | high | Q321 |
| low | Q322 | ||
| white | Q323 | ||
| MG | Q324 |
| Product | Description | Tube | Art. No. |
|---|---|---|---|
| GEN-IAL® QuickGEN Wine Screening without yeast | Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria | high | Q331 |
| low | Q332 | ||
| white | Q333 | ||
| MG | Q334 | ||
| GEN-IAL® Biogenic Amines | Specific DNA detection of bacteria forming biogenic amines | – | Q345 |
| Product | Description | Tube | Art. No. |
|---|---|---|---|
| GEN-IAL® QuickGEN Yeast Dekkera bruxellensis quantitative | Specific DNA detection of Dekkera bruxellensis | high | Q371 |
| low | Q372 | ||
| white | Q373 | ||
| GEN-IAL® Dekkera bruxellensis quantitative | Specific DNA detection of Dekkera bruxellensis (FAM/HEX) | – | Q395 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. | Specific DNA detection of Dekkera spp. | high | Q551 |
| low | Q552 | ||
| white | Q553 | ||
| – | Q555 | ||
| GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii | Specific DNA detection of Zygosaccharomyces bailii | high | Q561 |
| low | Q562 | ||
| white | Q563 | ||
| QuickGEN PCR Kit Zygosaccharomyces rouxii | Specific DNA detection of Zygosaccharomyces rouxii | high | Q581 |
| low | Q582 | ||
| white | Q583 | ||
| QuickGEN PCR Kit Yeast universal | Qualitative DNA detection of yeast | high | Q981 |
| low | Q982 | ||
| white | Q983 |
DNA preparation:
| Product | Description | Art. No. |
|---|---|---|
| GEN-IAL® Simplex® Easy Wine | DNA preparation of wine samples | Q300 |
| GEN-IAL® QuickGEN Sample Preparation Centrifugation | DNA preparation of beverage samples, centrifugation | Q002 |
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| QuickGEN PCR Kit Screening and differentiation of wine spoilers |
Screening and differentiation of 8 bacteria and yeast in wine. The following bacteria and yeasts are differentiated: Lactobacilli / Pediococci, Oenococcus oeni, Acetic acid bacteria, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Dekkera … Read more |
96 reactions / 24 samples | Q231, Q232, Q233 |
| QuickGEN PCR Kit Screening and differentiation of wine spoilers |
Screening and differentiation of 14 bacteria and yeast in wine. The following yeast are differentiated: Lactobacillus spp. Pediococcus spp. Acetic acid bacteria Leuconostoc mesenteroides Oenococcus oeni Lactococcus lactis Alicyclobacillus spp. … Read more |
12 samples / 96 reatcions | Q221, Q222, Q223 |
| QuickGEN PCR Kit Zygosaccharomyces rouxii |
Detection of Zygosaccharomyces rouxii in wine and grape must by real-time PCR. Read more |
48 reactions | Q583 |
| QuickGEN PCR Kit Yeast universal -low- |
Qualitative detection of yeast contaminations in beverages. For exapmple: Saccharomyces spp. Dekkera spp. Zygosaccharomyces spp. Rhodotorula spp. Torulaspora spp. Kluyveromyces spp. Debaromyces spp. Candida spp. Hanseniaspora spp. Pichia spp Read more |
48 reactions: 48 x Dye Strips (freeze-dried, incl. IC-DNA) | Q982 |
| GEN-IAL® Dekkera bruxellensis Standard DNA |
Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR. Read more |
1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried) | Q360 |
| GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white |
Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Read more |
48 reactions | Q563 |
| GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low |
Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Read more |
48 reactions (tubes incl. Lyticase and IC-DNA) | Q562 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. |
Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus. Read more |
50 reactions | Q555 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. white |
Detection of Dekkera spp. in wine and grape must by real-time PCR. Read more |
48 reactions | Q553 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. low |
Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus Read more |
48 reactions | Q552 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. high |
Detection of Dekkera spp. in wine and grape must by real-time PCR. Read more |
48 reactions | Q551 |
| GEN-IAL® QuickGEN Yeast differentiation white |
Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus … Read more |
96 reactions for 12 samples | Q543 |
| GEN-IAL® QuickGEN Yeast differentiation low |
Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus … Read more |
96 reactions for 12 samples (tubes contain lyticase). | Q542 |
| GEN-IAL® QuickGEN Wild yeast 2 low |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia … Read more |
48 reactions | Q532 |
| GEN-IAL® QuickGEN Wild yeast 1 |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila … Read more |
50 reactions | Q525 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/HEX |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Read more |
50 reactions | Q395 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/ROX |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Read more |
50 reactions | Q385 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative white |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Read more |
48 reactions | Q373 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative low |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Read more |
48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). | Q372 |
| GEN-IAL® QuickGEN Wine Screening without yeast low MG |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Read more |
48 reactions | Q334 |
| GEN-IAL® QuickGEN Wine Screening without yeast white |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Read more |
48 reactions | Q333 |
| GEN-IAL® QuickGEN Wine Screening without yeast low |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Read more |
48 reactions | Q332 |
| GEN-IAL® QuickGEN Wine Screening low MG |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control Read more |
48 reactions | Q324 |
| GEN-IAL® QuickGEN Wine Screening white |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control Read more |
48 reactions | Q323 |
| GEN-IAL® QuickGEN Wine Screening low |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Read more |
48 reactions | Q322 |
| GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high |
Detection of Saccharomyces diastaticus in beverages by real-time PCR. Read more |
48 reactions | Q181 |
| GEN-IAL® QuickGEN Wine Screening without yeast high |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Read more |
48 reactions | Q331 |
| GEN-IAL® QuickGEN Wine Screening high |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control Read more |
48 reactions | Q321 |
| GEN-IAL® QuickGEN Wild yeast 2 |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia … Read more |
50 reactions | Q535 |
| GEN-IAL® QuickGEN Wild yeast 1 low |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila … Read more |
48 reactions | Q522 |
| GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus low |
Detection of Saccharomyces diastaticus in beverages by real-time PCR. Read more |
48 reactions | Q182 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative high |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Read more |
48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). | Q371 |
| GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii high |
Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Read more |
48 reactions (tubes incl. Lyticase and IC-DNA) | Q561 |
| GEN-IAL® Simplex® Easy Wine |
Microbial DNA extraction from wine by centrifugation. Suitable for white wine, red wine, most etc.. Read more |
100 Extractions | Q300 |
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