Mycotoxins are toxic secondary metabolites produced by filamenous fungi, mainly Aspergillus, Fusarium, and Penicillium species. Detecting mycotoxins is essential to protect human and animal health, ensure food safety, comply with regulatory limits, and minimize economic losses.

Continuous monitoring using reliable analytical techniques is a fundamental component. Learn more about the full range of analytical options in the flyer “Mycotoxins: a potential hazard“.

R-Biopharm’s product portfolio for mycotoxin detection consists of test kits as ELISA (enzyme-linked immunosorbent assay) and Lateral flow device (LFD, lateral flow assay) as well as screening cards, immunoaffinity columns (IAC) and solid phase extraction columns (SPE).

Learn more:

• R-Biopharm Lateral Flow Assays: Rapid Tests for Food Analysis
• R-Biopharm and ELISA Tests: Efficient immunoassays for food analysis
• R-Biopharm Purification Columns: Efficient Sample Clean-up

ELISA test kits are competitive enzyme immunoassays based on an antigen-antibody reaction for the quantification of mycotoxins in samples.

LFD test kits are immunochromatographic tests in strip format for the quantification of mycotoxins in samples.

Learn more:

• R-Biopharm Lateral Flow Assays: Rapid Tests for Food Analysis
• R-Biopharm and ELISA Tests: Efficient immunoassays for food analysis
• R-Biopharm Purification Columns: Efficient Sample Clean-up

The product lines for ELISA in mycotoxin detection are:

RIDASCREEN®

• Microtiter plate with 96 determinations (including standards)
• Include 6 or 5 standards (known and increasing concentrations of the mycotoxin used to generate a calibration curve for quantifying the analyte in unknown samples)
• Most of the test kits have an incubation time of 45 minutes, depending on the matrix
• It can be performed manually using the RIDA®ABSORBANCE 96 (Art. No. ZRA96FF) as a compact reader and RIDASOFT® NET Food & Feed (Art. No. Z9996FF) as software-based analysis, or using a fully analysis automated with Thunderbolt® or Bolt®

RIDASCREEN®FAST

• Microtiter plate with 96 or 48 determinations (including standards)
• Include 6 or 5 standards (known and increasing concentrations of the mycotoxin used to generate a calibration curve for quantifying the analyte in unknown samples)
• Most of the test kits have an incubation time of 8 or 15 minutes, depending on the test kit and the matrix
• After the manually analysis, it is possible to use the RIDA®ABSORBANCE 96 (Art. No. ZRA96FF) as a compact reader and RIDASOFT®NET Food & Feed (Art. No. Z9996FF) as software-based analysis

RIDASCREEN®FAST SC

• Microtiter plate with 48 determinations (including standards)
• Include only 1 Standard (0 concentration of mycotoxin – the calibration curve is calculated using the RIDASOFT® NET software)
• The incubation time varies from 8 to 25 minutes, depending on the test kit and the matrix
• After the manually analysis, it is possible to use the RIDA®ABSORBANCE 96 (Art. No. ZRA96FF) as a compact reader and RIDASOFT®NET Food & Feed (Art. No. Z9996FF) as software-based analysis

The product line for LFD in mycotoxin detection is:

RIDA®QUICK

• In total, 20 test strips included in each test kit
• No calibration is needed
• The incubation time is 3 or 5 minutes, depending on the test kit and the matrix
• Results are evaluated with: the RIDA®SMART APP software (Art. No. ZRSAM) and an approved smartphone or the RIDA®SMART APP software (Art. No. ZRSAM) installed on an Android smartphone and the RIDA®SMART BOX (Art. No. ZRSA-SB)

ELISA test kits:

• RIDASCREEN® – Total aflatoxin, aflatoxin B1, aflatoxin M1, deoxynivalenol (DON), total fumonisin, ochratoxin A, T-2/HT-2 and zearalenone
• RIDASCREEN®FAST – Total aflatoxin, citrinin, deoxynivalenol (DON), total fumonisin, ochratoxin A, T-2 and zearalenone
• RIDASCREEN®FAST SC – Total aflatoxin, deoxynivalenol (DON) and zearalenone

For the LFD test kits:

• RIDA®QUICK – total aflatoxin, deoxynivalenol (DON), total fumonisin, ochratoxin A, T-2/HT-2 and zearalenone

The ELISA tests use aqueous extraction or methanol-based extraction to extract the mycotoxin from the sample, depending on the test kit.

The LFD tests use aqueous extraction, methanol, or/and ethanol-based extraction to extract the mycotoxin from the sample, depending on the test kit.

Yes, there is a common extraction option for the RIDASCREEN® and RIDASCREEN®FAST using the ECO Extractor (aqueous extraction) included in the following test kits:

For the RIDASCREEN® test kits:

• RIDASCREEN® Fumonisin (Art. No. R3411), RIDASCREEN® Ochratoxin A 30/15 (Art. No. R1312) and RIDASCREEN® Zearalenon ECO (Art. No. R1403)

For the RIDASCREEN®FAST test kits:

• RIDASCREEN®FAST Fumonisin ECO (Art. No. R5603) and RIDASCREEN®FAST Ochratoxin A (Art. No. R5402)

Learn more:

• RIDA®QUICK Mycotoxin ECO Extractor – Food & Feed Analysis

Yes, there are 2 options of common extraction for the RIDA®QUICK test kits:

1. Ethanol extraction:
   • RIDA®QUICK Aflatoxin RQS (Art. No. R5208)
   • RIDA®QUICK Zearalenon RQS (Art. No. R5504)

2. Aqueous extraction:
   • RIDA®QUICK Mycotoxin ECO Extractor (universal extraction buffer for RIDA®QUICK mycotoxin test kits, Art. No. R5000 – not included in the test kits)
   • RIDA®QUICK DON RQS ECO (Art. No. R5911)
   • RIDA®QUICK Fumonisin RQS ECO (Art. No. R5606)
   • RIDA®QUICK Ochratoxin ECO (Art. No. R5404)
   • RIDA®QUICK T-2/HT-2 RQS ECO (Art. No. R5304)

It is possible to use RIDASOFT® Win.NET Food & Feed (Art. No. Z9996FF). The software processes data which is broadcast by a serial interface. It is compatible with all quantitative ELISA mycotoxin tests of RIDASCREEN®, RIDASCREEN®FAST and RIDASCREEN®FAST SC.

For the evaluation of the LFD test kits (RIDA®QUICK), the RIDA®SMART APP Mycotoxin (Art. No. ZRSAM) is needed. The results are evaluated with an approved Android smartphone or an Android smartphone and the RIDA®SMART BOX (Art. No. ZRSA-SB). The test results can be shared via e-mail or printed as well as instantly uploaded to the cloud or RIDA®SMART Universe. The new RIDA®SMART Universe cloud-based data management solution offers efficient real-time data management from any device.