PCR en tiempo real
Diagnóstico molecular para análisis alimentario
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PCR en tiempo real
El ensayo de PCR (abreviatura de reacción en cadena de la polimerasa) es un importante método de análisis de los alimentos y de diagnóstico médico.
Los ensayos de PCR en tiempo real duplican y analizan secuencias de ADN específicas. En el análisis alimentario, los ensayos de PCR se utilizan en muchas aplicaciones, como la detección de microorganismos patogénicos, la identificación de alérgenos, la detección de organismos genéticamente modificados (GMO) o la identificación de especies animales. Los ensayos de PCR ofrecen numerosas ventajas: Son muy específicos, sensibles, sólidos, rápidos y fiables. Además, se pueden automatizar.
- Seguro: método específico y validado
- Fiable: kits de ensayo con control de calidad
- Fácil de usar: preparación de muestras intuitiva y universal
- Efectivo: capacidad para cuantificación y procesamiento múltiple
- Versátil: numerosas áreas de aplicación
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PCR en tiempo real explicada
En todos los análisis, el primer paso consiste en un muestreo representativo y una homogeneización. Tras la lisis de las células orgánicas del tejido de alimento, el ADN se preparará mediante diferentes kits de preparación de ADN y ARN específicos, ya sea para bacterias o para virus.
Los kits SureFood® PREP Basic y SureFood® Prep Advanced se utilizan para piensos y alimentos sólidos de origen animal o vegetal. En el caso de las bebidas, se utilizan kits de preparación de ADN específicos dependiendo de la matriz, mientras que la nueva gama de productos QuickGEN permite una manipulación sumamente sencilla.
El sistema modular de preparación del ADN y detección de ADN se puede utilizar de forma independiente. La amplificación del ADN de destino requiere, además de los kits apropiados, el uso de un termociclador adecuado con entre dos (FAM/HEX) y cuatro (FAM/HEX/ROX/Cy5) canales de detección.
Áreas de aplicación de PCR en el análisis de alimentos
Las alergias alimentarias pueden provocar reacciones alérgicas graves. Para proteger a los clientes, los productos alimentarios se deben analizar para determinar la presencia de sustancias alergénicas. Ofrecemos una amplia gama de kits de PCR para la detección de alérgenos como soja, avellana, cacahuete, gluten, almendra, apio, sésamo, mostaza, pescado o crustáceos. También ofrecemos ensayos múltiples para detección simultánea de varios alérgenos (cacahuete/avellana/nuez y apio/soja/mostaza).
La especificación de la especie es de interés especial: los productos cárnicos se pueden falsificar por medio de una declaración incorrecta (como en el escándalo de la carne de caballo). Otra de sus aplicaciones es la garantía del cumplimiento de prescripciones dietéticas de carácter religioso (halal, kosher). Nuestra gama de productos SureFood® Animal ID permite un análisis de mayor sensibilidad de muchos tipos de carne (cerdo, ternera, caballo, asno, pollo, pavo y otras especies). Con la línea de productos SureFood® Fish ID, ofrecemos una amplia gama de kits de PCR para la identificación de especies ícticas.
Durante la producción de bebidas, la detección de elementos que favorecen el deterioro es crucial. Con la línea de productos GEN-IAL®, ofrecemos una amplia gama de kits de cribado para la detección de organismos que causan el deterioro durante la producción de cerveza y vino, por ejemplo, Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis o Oenococcus oeni. Los kits de ensayo se pueden utilizar tanto para control durante el proceso como para control del producto final.
En años recientes, la producción mundial de alimentos modificados genéticamente se ha incrementado paulatinamente. Desde que se estableció el etiquetado obligatorio de los productos alimentarios que contienen organismos modificados genéticamente (GMO), la demanda de métodos de ensayo de alta sensibilidad se ha incrementado. Nuestra gama de productos SureFood® GMO permite la detección cuantitativa y cualitativa de GMO, como maíz modificado genéticamente, arroz LibertyLink o soja Roundup Ready.
La detección de la contaminación microbiológica es uno de los principales campos de aplicación del PCR en análisis alimentario. Nuestra gama de productos SureFast® detecta una amplia gama de bacterias patogénicas (por ejemplo, Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), virus (por ejemplo, norovirus, hepatitis y gripe), así como levaduras y moho, en los alimentos.
Descripción de productos de PCR en tiempo real
Encontrará un amplio resumen de los kits de ensayo de PCR SureFood® / SureFast® en nuestro folleto gratuito.
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más |
96 preparations | F1060 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Lee más |
100 reactions | S7007 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® ALLERGEN 4plex Almond/Pistachio/Cashew+IAC |
The SureFood® ALLERGEN 4plex Almond/Pistachio/Cashew+IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific almond (Prunus dulcis), pistachio (Pistacia vera) and cashew (Anacardium occidentale) DNA sequences … Lee más |
100 reactions | S3406 |
| SureFood® ALLERGEN 4plex EU NUTS |
The SureFood® ALLERGEN 4plex EU NUTS is a real-time PCR for the direct, qualitative detection and differentiation of almond (Prunus dulcis), cashew (Anacardium occidentale), pistachio (Pistacia vera), peanut (Arachis hypogaea), hazelnut (Corylus … Lee más |
100 reactions | S3404 |
| SureFood® ALLERGEN Shea Nut |
The SureFood® ALLERGEN Shea Nut is a real-time PCR for the direct, qualitative detection of specific shea nut (Vitellaria paradoxa) DNA sequences in raw material. Lee más |
100 reactions | S3622 |
| SureFood® ALLERGEN Beechnut |
The SureFood® ALLERGEN Beechnut is a real-time PCR for the direct qualitative detection of specific beechnut (Fagus sylvatica) DNA sequences in food. Lee más |
100 reactions | S3628 |
| SureFood® ALLERGEN Insects |
The SureFood® ALLERGEN Insects is a real-time PCR for the direct, qualitative detection of specific insects (Insecta) DNA sequences. Lee más |
100 reactions | S3626 |
| SureFood® ALLERGEN Pine Nut |
The SureFood® ALLERGEN Pine Nut is a real-time PCR for the direct, qualitative detection of specific pine nut (Pinus spp.) DNA sequences in food. Each reaction contains an internal amplification control (IAC). Lee más |
100 reactions | S3624 |
| SureFood® ALLERGEN Buckwheat |
The SureFood® ALLERGEN Buckwheat is a real-time PCR for the direct, qualitative and / or quantitative detection of specific buckwheat (Fagopyrum esculentum) DNA sequences. For the quantitative determination the use of the laboratory reference … Lee más |
100 reactions | S3620 |
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más |
96 preparations | F1060 |
| SureFood® ALLERGEN 4plex Cereals |
The SureFood® ALLERGEN 4plex Cereals is a real-time PCR test for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences in food. Each reaction contains an … Lee más |
100 reactions | S7006 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Lee más |
100 reactions | S7007 |
| SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC |
The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya … Lee más |
100 reactions | S3403 |
| SureFood® Oat |
The real-time PCR test detects DNA of oat (Avena sativa) qualitatively. Each reaction contains an internal amplification control (IAC). Lee más |
100 reactions | S7004 |
| SureFood® ALLERGEN Mustard |
The SureFood® ALLERGEN Mustard is a real-time PCR for the direct, qualitative and / or quantitative detection of specific white mustard (Sinapis alba), indian mustard (Brassica juncea) und black mustard (Brassica nigra) DNA sequences according to … Lee más |
100 reactions | S3609 |
| SureFood® ALLERGEN Lupin |
The real-time PCR test detects DNA of lupin (Lupinus spp.) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of the … Lee más |
100 reactions | S3611 |
| SureFood® ALLERGEN Celery |
The real-time PCR test detects DNA of celery (Apium graveolens) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Lee más |
100 reactions | S3605 |
| SureFood® ALLERGEN Molluscs |
The SureFood® ALLERGEN Molluscs is a real-time PCR for the direct, qualitative and / or quantitative detection of specific molluscs (mollusca) DNA sequences according to directive (EC) 1169/2011 in food. The molluscs family contains 50,000 – … Lee más |
100 reactions | S3613 |
| SureFood® ALLERGEN Fish |
The SureFood® ALLERGEN Fish is a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large … Lee más |
100 reactions | S3610 |
| SureFood® ALLERGEN Crustaceans |
The SureFood® ALLERGEN Crustaceans is a real-time PCR for the direct, qualitative and / or quantitative detection of specific crustaceans (Crustacea) DNA sequences according to directive (EC) 1169/2011. The crustacean family contains about 44,000 … Lee más |
100 reactions | S3612 |
| SureFood® ALLERGEN Sesame |
The real-time PCR test detects DNA of sesame (Sesamum indicum) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Lee más |
100 reactions | S3608 |
| SureFood® ALLERGEN Walnut |
The SureFood® ALLERGEN Walnut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific walnut (Juglans regia and Juglans nigra) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination … Lee más |
100 reactions | S3607 |
| SureFood® ALLERGEN Pistachio |
The SureFood® ALLERGEN Pistachio is a real-time PCR for the direct, qualitative and / or quantitative detection of specific pistachio (Pistacia vera) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination the use of … Lee más |
100 reactions | S3614 |
| SureFood® ALLERGEN Pecan |
The SureFood® ALLERGEN Pecan is a real-time PCR for the direct, qualitative and / or quantitative detection of specific pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination the use of … Lee más |
100 reactions | S3618 |
| SureFood® ALLERGEN Peanut |
The real-time PCR test detects DNA of peanut (Arachis hypogaea) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Lee más |
100 reactions | S3603 |
| SureFood® ALLERGEN Macadamia |
The real-time PCR test detects DNA of macadamia (Macadamia ternifolia) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the … Lee más |
100 reactions | S3616 |
| SureFood® ALLERGEN Hazelnut |
The real-time PCR test detects DNA of hazelnut (Glycine max) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Lee más |
100 reactions | S3602 |
| SureFood® ALLERGEN Cashew |
The real-time PCR test detects DNA of cashew (Anacardium occidentale) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the … Lee más |
100 reactions | S3615 |
| SureFood® ALLERGEN Brazil Nut |
The SureFood® ALLERGEN Brazil Nut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific brazil nut (Bertholletia excelsa) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination … Lee más |
100 reactions | S3617 |
| SureFood® ALLERGEN Almond |
The real-time PCR test detects DNA of almond (Prunus dulcis) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Lee más |
100 reactions | S3604 |
| SureFood® ALLERGEN Soya |
The real-time PCR test detects soya DNA (Glycine max) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of the … Lee más |
100 reactions | S3601 |
| SureFood® ALLERGEN Gluten |
The SureFood® ALLERGEN Gluten is a real-time PCR for the direct, qualitative and / or quantitative detection of specific gluten-containing cereals (wheat such as spelt and khorasan wheat, rye, barley, oats) DNA sequences according to directive (EC) … Lee más |
100 reactions | S3606 |
| SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC |
The SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific celery (Apium graveolens), mustard (Brassica carinata, Brassica juncea, Brassica nigra, Sinapis alba, … Lee más |
100 reactions | S3401 |
| SureFood® QUANTARD Allergen 40 |
SureFood® QUANTARD Allergen 40 is a laboratory reference material developed for quantification in the range of 1 – 400 mg allergenic substance / kg food. Lee más |
1x 2 g | S3301 |
| SureFood® ALLERGEN 4plex Peanut / Hazelnut / Walnut + IAC |
The SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific Peanut (Arachis hypogaea), Hazelnut (Corylus maxima) and Walnut (Juglans regia, Juglans nigra) DNA … Lee más |
100 reactions | S3402 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más |
96 preparations | F1060 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más |
96 preparations | F1060 |
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