PCR en tiempo real
Diagnóstico molecular para análisis alimentario
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PCR en tiempo real
El ensayo de PCR (abreviatura de reacción en cadena de la polimerasa) es un importante método de análisis de los alimentos y de diagnóstico médico.
Los ensayos de PCR en tiempo real duplican y analizan secuencias de ADN específicas. En el análisis alimentario, los ensayos de PCR se utilizan en muchas aplicaciones, como la detección de microorganismos patogénicos, la identificación de alérgenos, la detección de organismos genéticamente modificados (GMO) o la identificación de especies animales. Los ensayos de PCR ofrecen numerosas ventajas: Son muy específicos, sensibles, sólidos, rápidos y fiables. Además, se pueden automatizar.
- Seguro: método específico y validado
- Fiable: kits de ensayo con control de calidad
- Fácil de usar: preparación de muestras intuitiva y universal
- Efectivo: capacidad para cuantificación y procesamiento múltiple
- Versátil: numerosas áreas de aplicación
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PCR en tiempo real explicada
En todos los análisis, el primer paso consiste en un muestreo representativo y una homogeneización. Tras la lisis de las células orgánicas del tejido de alimento, el ADN se preparará mediante diferentes kits de preparación de ADN y ARN específicos, ya sea para bacterias o para virus.
Los kits SureFood® PREP Basic y SureFood® Prep Advanced se utilizan para piensos y alimentos sólidos de origen animal o vegetal. En el caso de las bebidas, se utilizan kits de preparación de ADN específicos dependiendo de la matriz, mientras que la nueva gama de productos QuickGEN permite una manipulación sumamente sencilla.
El sistema modular de preparación del ADN y detección de ADN se puede utilizar de forma independiente. La amplificación del ADN de destino requiere, además de los kits apropiados, el uso de un termociclador adecuado con entre dos (FAM/HEX) y cuatro (FAM/HEX/ROX/Cy5) canales de detección.
Áreas de aplicación de PCR en el análisis de alimentos
Las alergias alimentarias pueden provocar reacciones alérgicas graves. Para proteger a los clientes, los productos alimentarios se deben analizar para determinar la presencia de sustancias alergénicas. Ofrecemos una amplia gama de kits de PCR para la detección de alérgenos como soja, avellana, cacahuete, gluten, almendra, apio, sésamo, mostaza, pescado o crustáceos. También ofrecemos ensayos múltiples para detección simultánea de varios alérgenos (cacahuete/avellana/nuez y apio/soja/mostaza).
La especificación de la especie es de interés especial: los productos cárnicos se pueden falsificar por medio de una declaración incorrecta (como en el escándalo de la carne de caballo). Otra de sus aplicaciones es la garantía del cumplimiento de prescripciones dietéticas de carácter religioso (halal, kosher). Nuestra gama de productos SureFood® Animal ID permite un análisis de mayor sensibilidad de muchos tipos de carne (cerdo, ternera, caballo, asno, pollo, pavo y otras especies). Con la línea de productos SureFood® Fish ID, ofrecemos una amplia gama de kits de PCR para la identificación de especies ícticas.
Durante la producción de bebidas, la detección de elementos que favorecen el deterioro es crucial. Con la línea de productos GEN-IAL®, ofrecemos una amplia gama de kits de cribado para la detección de organismos que causan el deterioro durante la producción de cerveza y vino, por ejemplo, Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis o Oenococcus oeni. Los kits de ensayo se pueden utilizar tanto para control durante el proceso como para control del producto final.
En años recientes, la producción mundial de alimentos modificados genéticamente se ha incrementado paulatinamente. Desde que se estableció el etiquetado obligatorio de los productos alimentarios que contienen organismos modificados genéticamente (GMO), la demanda de métodos de ensayo de alta sensibilidad se ha incrementado. Nuestra gama de productos SureFood® GMO permite la detección cuantitativa y cualitativa de GMO, como maíz modificado genéticamente, arroz LibertyLink o soja Roundup Ready.
La detección de la contaminación microbiológica es uno de los principales campos de aplicación del PCR en análisis alimentario. Nuestra gama de productos SureFast® detecta una amplia gama de bacterias patogénicas (por ejemplo, Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), virus (por ejemplo, norovirus, hepatitis y gripe), así como levaduras y moho, en los alimentos.
Descripción de productos de PCR en tiempo real
Encontrará un amplio resumen de los kits de ensayo de PCR SureFood® / SureFast® en nuestro folleto gratuito.
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of … Lee más |
96 preparations | F1060 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of … Lee más |
96 preparations | F1060 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| QuickGEN PCR Kit Yeast universal -low- |
Qualitative detection of yeast contaminations in beverages. For exapmple: Saccharomyces spp. Dekkera spp. Zygosaccharomyces spp. Rhodotorula spp. Torulaspora spp. Kluyveromyces spp. Debaromyces spp. Candida spp. Hanseniaspora spp. Pichia spp Lee más |
48 reactions: 48 x Dye Strips (freeze-dried, incl. IC-DNA) | Q982 |
| GEN-IAL® QuickGEN P1 and Dekkera spp. Screening low MG |
Qualitative detection of bacterial and Dekkera spp. contaminations in filtrated beer, beer mixes and yeast containing samples. Lee más |
48 reactions | Q094 |
| SureFast® Listeria 3plex ONE |
The SureFast® Listeria 3plex ONE can be applied for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food. The method consists of the following three steps: 1) Cultural enrichment 2) … Lee más |
100 reactions | F5217 |
| QuickGEN PCR Kit Fusarium spp. |
Detection of Fusarium spp. in beverages, cereals etc. by real-time PCR. Lee más |
48 reactions | Q961 |
| QuickGEN Pentaplex PCR Kit – Beer spoilage bacteria and yeast screening |
Qualitative detection of bacterial, S. cerevisiae var. diastaticus and Dekkera spp. contaminations in filtered beer, beer mixes and yeast containing samples. Lee más |
48 reactions | Q061 |
| SureFast® Campylobacter 4plex |
The SureFast® Campylobacter 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Campylobacter jejuni, Campylobacter lari and Campylobacter coli. Lee más |
100 reactions | F5170 |
| SureFast® Mag PREP Pathogens |
The kit is intended to be used for the isolation of DNA and RNA viruses nucleic acids from food and feed (e.g. wash up fluids from fruits, salads etc.), filters from water samples and swabs (e.g. from work surfaces etc.) as well as bacterial DNA from … Lee más |
96 preparations | F1062 |
| SureFast® STEC 4plex ONE |
The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli … Lee más |
100 reactions | F5265 |
| SureFast® MRSA 4plex |
The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of … Lee más |
100 reactions | F7117 |
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® SARS-CoV-2 PLUS |
The SureFast® SARS-CoV-2 PLUS is a real-time RT-PCR for the direct, qualitative detection of novel coronavirus (SARS-CoV-2) RNA. Each reaction contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample … Lee más |
100 reactions | F7110 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of … Lee más |
96 preparations | F1060 |
| GEN-IAL® QuickGEN PCR Kit Alicyclobacillus Differentiation high |
Qualitative detection and differentiation of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris . Lee más |
48 reactions | Q721 |
| SureFast® Yersinia 3plex |
The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica. Lee más |
100 reactions | F5132 |
| GEN-IAL® Dekkera bruxellensis Standard DNA |
Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR. Lee más |
1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried) | Q360 |
| GEN-IAL® QuickGEN PCR Kit S. cerevisiae var. diastaticus white |
Detection of S. cerevisiae var. diastaticus in beverages. Lee más |
48 reactions | Q183 |
| GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white |
Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Lee más |
48 reactions | Q563 |
| GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low |
Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Lee más |
48 reactions (tubes incl. Lyticase and IC-DNA) | Q562 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. |
Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus. Lee más |
50 reactions | Q555 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. white |
Detection of Dekkera spp. in wine and grape must by real-time PCR. Lee más |
48 reactions | Q553 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. low |
Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus Lee más |
48 reactions | Q552 |
| GEN-IAL® QuickGEN Yeast Dekkera spp. high |
Detection of Dekkera spp. in wine and grape must by real-time PCR. Lee más |
48 reactions | Q551 |
| GEN-IAL® QuickGEN Yeast differentiation white |
Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus … Lee más |
96 reactions for 12 samples | Q543 |
| GEN-IAL® QuickGEN Yeast differentiation low |
Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus … Lee más |
96 reactions for 12 samples (tubes contain lyticase). | Q542 |
| GEN-IAL® QuickGEN Wild yeast 2 low |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia … Lee más |
48 reactions | Q532 |
| GEN-IAL® QuickGEN Wild yeast 1 |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila … Lee más |
50 reactions | Q525 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/HEX |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Lee más |
50 reactions | Q395 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/ROX |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Lee más |
50 reactions | Q385 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative white |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Lee más |
48 reactions | Q373 |
| GEN-IAL® QuickGEN Dekkera bruxellensis quantitative low |
Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must. Lee más |
48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). | Q372 |
| GEN-IAL® QuickGEN Wine Screening without yeast low MG |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Lee más |
48 reactions | Q334 |
| GEN-IAL® QuickGEN Wine Screening without yeast white |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Lee más |
48 reactions | Q333 |
| GEN-IAL® QuickGEN Wine Screening without yeast low |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Lee más |
48 reactions | Q332 |
| GEN-IAL® QuickGEN Wine Screening low MG |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Lee más |
48 reactions | Q324 |
| GEN-IAL® QuickGEN Wine Screening white |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Lee más |
48 reactions | Q323 |
| GEN-IAL® QuickGEN Wine Screening low |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Lee más |
48 reactions | Q322 |
| GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high |
Detection of Saccharomyces diastaticus in beverages by real-time PCR. Lee más |
48 reactions | Q181 |
| GEN-IAL® QuickGEN Yeast Bottom fermented white |
Detection of bottom-fermented yeast in beverages by real-time PCR. Lee más |
48 reactions | Q163 |
| GEN-IAL® QuickGEN Yeast Bottom fermented low |
Detection of bottom-fermented yeast in beverages (e.g. beer, wine, grape must) by real-time PCR. Lee más |
48 reactions | Q162 |
| GEN-IAL® QuickGEN® Yeast Top fermented white |
Real-time PCR-Kit for the detection of top-fermented yeast. Lee más |
48 reactions | Q153 |
| GEN-IAL® QuickGEN® Yeast Top fermented low |
Real-time PCR-Kit for the detection of top-fermented yeast. Lee más |
48 reactions | Q152 |
| GEN-IAL® QuickGEN Beer differentiation white |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. … Lee más |
12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. | Q083 |
| GEN-IAL® QuickGEN Beer differentiation low |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. … Lee más |
12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. | Q082 |
| GEN-IAL® QuickGEN Beer yeast and bacteria differentiation white |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild … Lee más |
96 PCR reactions (24 samples) 12 stripes (tubes 2, 3, 5 and 6 contain lyticase) |
Q073 |
| GEN-IAL® QuickGEN Beer yeast and bacteria differentiation low |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild … Lee más |
96 PCR reactions (24 samples) 12 stripes |
Q072 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Lee más |
50 reactions | Q055 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance low MG |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Lee más |
48 reactions | Q054 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance white |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Lee más |
48 reactions | Q053 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of … Lee más |
96 preparations | F1060 |
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