PCR en tiempo real

Diagnóstico molecular para análisis alimentario

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PCR en tiempo real

El ensayo de PCR (abreviatura de reacción en cadena de la polimerasa) es un importante método de análisis de los alimentos y de diagnóstico médico.

Los ensayos de PCR en tiempo real duplican y analizan secuencias de ADN específicas. En el análisis alimentario, los ensayos de PCR se utilizan en muchas aplicaciones, como la detección de microorganismos patogénicos, la identificación de alérgenos, la detección de organismos genéticamente modificados (GMO) o la identificación de especies animales. Los ensayos de PCR ofrecen numerosas ventajas: Son muy específicos, sensibles, sólidos, rápidos y fiables. Además, se pueden automatizar.

  • Seguro: método específico y validado
  • Fiable: kits de ensayo con control de calidad
  • Fácil de usar: preparación de muestras intuitiva y universal
  • Efectivo: capacidad para cuantificación y procesamiento múltiple
  • Versátil: numerosas áreas de aplicación
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PCR en tiempo real explicada

En todos los análisis, el primer paso consiste en un muestreo representativo y una homogeneización. Tras la lisis de las células orgánicas del tejido de alimento, el ADN se preparará mediante diferentes kits de preparación de ADN y ARN específicos, ya sea para bacterias o para virus.

Los kits SureFood® PREP Basic y SureFood® Prep Advanced se utilizan para piensos y alimentos sólidos de origen animal o vegetal. En el caso de las bebidas, se utilizan kits de preparación de ADN específicos dependiendo de la matriz, mientras que la nueva gama de productos QuickGEN permite una manipulación sumamente sencilla.

El sistema modular de preparación del ADN y detección de ADN se puede utilizar de forma independiente. La amplificación del ADN de destino requiere, además de los kits apropiados, el uso de un termociclador adecuado con entre dos (FAM/HEX) y cuatro (FAM/HEX/ROX/Cy5) canales de detección.

Áreas de aplicación de PCR en el análisis de alimentos

Las alergias alimentarias pueden provocar reacciones alérgicas graves. Para proteger a los clientes, los productos alimentarios se deben analizar para determinar la presencia de sustancias alergénicas. Ofrecemos una amplia gama de kits de PCR para la detección de alérgenos como soja, avellana, cacahuete, gluten, almendra, apio, sésamo, mostaza, pescado o crustáceos. También ofrecemos ensayos múltiples para detección simultánea de varios alérgenos (cacahuete/avellana/nuez y apio/soja/mostaza).

La especificación de la especie es de interés especial: los productos cárnicos se pueden falsificar por medio de una declaración incorrecta (como en el escándalo de la carne de caballo). Otra de sus aplicaciones es la garantía del cumplimiento de prescripciones dietéticas de carácter religioso (halal, kosher). Nuestra gama de productos SureFood® Animal ID permite un análisis de mayor sensibilidad de muchos tipos de carne (cerdo, ternera, caballo, asno, pollo, pavo y otras especies). Con la línea de productos SureFood® Fish ID, ofrecemos una amplia gama de kits de PCR para la identificación de especies ícticas.

Durante la producción de bebidas, la detección de elementos que favorecen el deterioro es crucial. Con la línea de productos GEN-IAL®, ofrecemos una amplia gama de kits de cribado para la detección de organismos que causan el deterioro durante la producción de cerveza y vino, por ejemplo, Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis o Oenococcus oeni. Los kits de ensayo se pueden utilizar tanto para control durante el proceso como para control del producto final.

En años recientes, la producción mundial de alimentos modificados genéticamente se ha incrementado paulatinamente. Desde que se estableció el etiquetado obligatorio de los productos alimentarios que contienen organismos modificados genéticamente (GMO), la demanda de métodos de ensayo de alta sensibilidad se ha incrementado. Nuestra gama de productos SureFood® GMO permite la detección cuantitativa y cualitativa de GMO, como maíz modificado genéticamente, arroz LibertyLink o soja Roundup Ready.

La detección de la contaminación microbiológica es uno de los principales campos de aplicación del PCR en análisis alimentario. Nuestra gama de productos SureFast® detecta una amplia gama de bacterias patogénicas (por ejemplo, Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), virus (por ejemplo, norovirus, hepatitis y gripe), así como levaduras y moho, en los alimentos.

Descripción de productos de PCR en tiempo real

Encontrará un amplio resumen de los kits de ensayo de PCR SureFood® / SureFast® en nuestro folleto gratuito.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

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Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

Lee más
96 preparations F1060
TANBead Maelstrom™ 8 Autostage

The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

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Up to 8 samples ZMAL8
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ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Lee más
Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

Lee más
96 preparations F1060
TANBead Maelstrom™ 8 Autostage

The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

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Up to 8 samples ZMAL8
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ProductDescriptionNo. of tests/amountArt. No.
SureFast® Listeria 3plex ONE

The SureFast® Listeria 3plex ONE can be applied for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food. The method consists of the following three steps: 1) Cultural enrichment 2) …

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100 reactions F5217
QuickGEN PCR Kit Fusarium spp.

Detection of Fusarium spp. in beverages, cereals etc. by real-time PCR.

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48 reactions Q961
QuickGEN Pentaplex PCR Kit – Beer spoilage bacteria and yeast screening

Qualitative detection of bacterial, S. cerevisiae var. diastaticus and Dekkera spp. contaminations in filtered beer, beer mixes and yeast containing samples.

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48 reactions Q061
SureFast® Campylobacter 4plex

The SureFast® Campylobacter 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Campylobacter jejuni, Campylobacter lari and Campylobacter coli.

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100 reactions F5170
SureFast® Mag PREP Pathogens

The kit is intended to be used for the isolation of DNA and RNA viruses nucleic acids from food and feed (e.g. wash up fluids from fruits, salads etc.), filters from water samples and swabs (e.g. from work surfaces etc.) as well as bacterial DNA from …

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96 preparations F1062
SureFast® STEC 4plex ONE

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli …

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100 reactions F5265
SureFast® MRSA 4plex

The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of …

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100 reactions F7117
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Lee más
Up to 48 samples ZMAL48
SureFast® SARS-CoV-2 PLUS

The SureFast® SARS-CoV-2 PLUS is a real-time RT-PCR for the direct, qualitative detection of novel coronavirus (SARS-CoV-2) RNA. Each reaction contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample …

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100 reactions F7110
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

Lee más
96 preparations F1060
TANBead Maelstrom™ 8 Autostage

The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

Lee más
Up to 8 samples ZMAL8
GEN-IAL® QuickGEN PCR Kit Alicyclobacillus Differentiation high

Qualitative detection and differentiation of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris .

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48 reactions Q721
SureFast® Yersinia 3plex

The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica.

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100 reactions F5132
GEN-IAL® Dekkera bruxellensis Standard DNA

Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR.

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1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried) Q360
GEN-IAL® QuickGEN PCR Kit S. cerevisiae var. diastaticus white

Detection of S. cerevisiae var. diastaticus in beverages.

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48 reactions Q183
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions Q563
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions (tubes incl. Lyticase and IC-DNA) Q562
GEN-IAL® QuickGEN Yeast Dekkera spp.

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus.

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50 reactions Q555
GEN-IAL® QuickGEN Yeast Dekkera spp. white

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q553
GEN-IAL® QuickGEN Yeast Dekkera spp. low

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus

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48 reactions Q552
GEN-IAL® QuickGEN Yeast Dekkera spp. high

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q551
GEN-IAL® QuickGEN Yeast differentiation white

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples Q543
GEN-IAL® QuickGEN Yeast differentiation low

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples (tubes contain lyticase). Q542
GEN-IAL® QuickGEN Wild yeast 2 low

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia …

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48 reactions Q532
GEN-IAL® QuickGEN Wild yeast 1

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila …

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50 reactions Q525
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/HEX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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50 reactions Q395
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/ROX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

Lee más
50 reactions Q385
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative white

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions Q373
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative low

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). Q372
GEN-IAL® QuickGEN Wine Screening without yeast low MG

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q334
GEN-IAL® QuickGEN Wine Screening without yeast white

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q333
GEN-IAL® QuickGEN Wine Screening without yeast low

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

Lee más
48 reactions Q332
GEN-IAL® QuickGEN Wine Screening low MG

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Lee más
48 reactions Q324
GEN-IAL® QuickGEN Wine Screening white

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Lee más
48 reactions Q323
GEN-IAL® QuickGEN Wine Screening low

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Lee más
48 reactions Q322
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high

Detection of Saccharomyces diastaticus in beverages by real-time PCR.

Lee más
48 reactions Q181
GEN-IAL® QuickGEN Yeast Bottom fermented white

Detection of bottom-fermented yeast in beverages by real-time PCR.

Lee más
48 reactions Q163
GEN-IAL® QuickGEN Yeast Bottom fermented low

Detection of bottom-fermented yeast in beverages (e.g. beer, wine, grape must) by real-time PCR.

Lee más
48 reactions Q162
GEN-IAL® QuickGEN® Yeast Top fermented white

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q153
GEN-IAL® QuickGEN® Yeast Top fermented low

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q152
GEN-IAL® QuickGEN Beer differentiation white

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q083
GEN-IAL® QuickGEN Beer differentiation low

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q082
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation white

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

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96 PCR reactions (24 samples)
12 stripes (tubes 2, 3, 5 and 6 contain lyticase)
Q073
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation low

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

Lee más
96 PCR reactions (24 samples)
12 stripes
Q072
GEN-IAL® QuickGEN P1 Screening and Hop resistance

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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50 reactions Q055
GEN-IAL® QuickGEN P1 Screening and Hop resistance low MG

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q054
GEN-IAL® QuickGEN P1 Screening and Hop resistance white

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q053
GEN-IAL® QuickGEN P1 Screening and Hop resistance low

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

Lee más
48 reactions Q052
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ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Lee más
Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

Lee más
96 preparations F1060
TANBead Maelstrom™ 8 Autostage

The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

Lee más
Up to 8 samples ZMAL8
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