PCR en tiempo real
Diagnóstico molecular para análisis alimentario

PCR en tiempo real

El ensayo de PCR (abreviatura de reacción en cadena de la polimerasa) es un importante método de análisis de los alimentos y de diagnóstico médico.

Los ensayos de PCR en tiempo real duplican y analizan secuencias de ADN específicas. En el análisis alimentario, los ensayos de PCR se utilizan en muchas aplicaciones, como la detección de microorganismos patogénicos, la identificación de alérgenos, la detección de organismos genéticamente modificados (GMO) o la identificación de especies animales. Los ensayos de PCR ofrecen numerosas ventajas: Son muy específicos, sensibles, sólidos, rápidos y fiables. Además, se pueden automatizar.

Seguro: método específico y validado
Fiable: kits de ensayo con control de calidad
Fácil de usar: preparación de muestras intuitiva y universal
Efectivo: capacidad para cuantificación y procesamiento múltiple
Versátil: numerosas áreas de aplicación

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PCR en tiempo real explicada

En todos los análisis, el primer paso consiste en un muestreo representativo y una homogeneización. Tras la lisis de las células orgánicas del tejido de alimento, el ADN se preparará mediante diferentes kits de preparación de ADN y ARN específicos, ya sea para bacterias o para virus.

Los kits SureFood® PREP Basic y SureFood® Prep Advanced se utilizan para piensos y alimentos sólidos de origen animal o vegetal. En el caso de las bebidas, se utilizan kits de preparación de ADN específicos dependiendo de la matriz, mientras que la nueva gama de productos QuickGEN permite una manipulación sumamente sencilla.

El sistema modular de preparación del ADN y detección de ADN se puede utilizar de forma independiente. La amplificación del ADN de destino requiere, además de los kits apropiados, el uso de un termociclador adecuado con entre dos (FAM/HEX) y cuatro (FAM/HEX/ROX/Cy5) canales de detección.

Áreas de aplicación de PCR en el análisis de alimentos

Las alergias alimentarias pueden provocar reacciones alérgicas graves. Para proteger a los clientes, los productos alimentarios se deben analizar para determinar la presencia de sustancias alergénicas. Ofrecemos una amplia gama de kits de PCR para la detección de alérgenos como soja, avellana, cacahuete, gluten, almendra, apio, sésamo, mostaza, pescado o crustáceos. También ofrecemos ensayos múltiples para detección simultánea de varios alérgenos (cacahuete/avellana/nuez y apio/soja/mostaza).

La especificación de la especie es de interés especial: los productos cárnicos se pueden falsificar por medio de una declaración incorrecta (como en el escándalo de la carne de caballo). Otra de sus aplicaciones es la garantía del cumplimiento de prescripciones dietéticas de carácter religioso (halal, kosher). Nuestra gama de productos SureFood® Animal ID permite un análisis de mayor sensibilidad de muchos tipos de carne (cerdo, ternera, caballo, asno, pollo, pavo y otras especies). Con la línea de productos SureFood® Fish ID, ofrecemos una amplia gama de kits de PCR para la identificación de especies ícticas.

Durante la producción de bebidas, la detección de elementos que favorecen el deterioro es crucial. Con la línea de productos GEN-IAL®, ofrecemos una amplia gama de kits de cribado para la detección de organismos que causan el deterioro durante la producción de cerveza y vino, por ejemplo, Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis o Oenococcus oeni. Los kits de ensayo se pueden utilizar tanto para control durante el proceso como para control del producto final.

En años recientes, la producción mundial de alimentos modificados genéticamente se ha incrementado paulatinamente. Desde que se estableció el etiquetado obligatorio de los productos alimentarios que contienen organismos modificados genéticamente (GMO), la demanda de métodos de ensayo de alta sensibilidad se ha incrementado. Nuestra gama de productos SureFood® GMO permite la detección cuantitativa y cualitativa de GMO, como maíz modificado genéticamente, arroz LibertyLink o soja Roundup Ready.

La detección de la contaminación microbiológica es uno de los principales campos de aplicación del PCR en análisis alimentario. Nuestra gama de productos SureFast® detecta una amplia gama de bacterias patogénicas (por ejemplo, Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), virus (por ejemplo, norovirus, hepatitis y gripe), así como levaduras y moho, en los alimentos.

Descripción de productos de PCR en tiempo real

Encontrará un amplio resumen de los kits de ensayo de PCR SureFood® / SureFast® en nuestro folleto gratuito.

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Product portfolio

Adulteración de alimentos

Métodos analíticos modernos para detección de fraudes alimentarios

Producto	Descripción	No. of tests/amount	Art. No.
TANBead Maelstrom™ 4800	The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más	Up to 48 samples	ZMAL48
SureFast® Mag PREP Food	The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más	96 preparations	F1060

Alérgenos alimentarios

Soluciones analíticas de alta fiabilidad para control de alérgenos

Producto	Descripción	No. of tests/amount	Art. No.
TANBead Maelstrom™ 4800	The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más	Up to 48 samples	ZMAL48
SureFast® Mag PREP Food	The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más	96 preparations	F1060

Microbiología

Kits de ensayo para la detección de contaminación microbiológica

Producto	Descripción	No. of tests/amount	Art. No.
QuickGEN PCR Kit Screening and differentiation of wine spoilers	Screening and differentiation of 8 bacteria and yeast in wine. The following bacteria and yeasts are differentiated: Lactobacilli / Pediococci, Oenococcus oeni, Acetic acid bacteria, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Dekkera … Lee más	96 reactions / 24 samples	Q231, Q232, Q233
QuickGEN PCR Kit Screening and differentiation of wine spoilers	Screening and differentiation of 14 bacteria and yeast in wine. The following yeast are differentiated: Lactobacillus spp. Pediococcus spp. Acetic acid bacteria Leuconostoc mesenteroides Oenococcus oeni Lactococcus lactis Alicyclobacillus spp. … Lee más	12 samples / 96 reatcions	Q221, Q222, Q223
Simplex® Easy Spin Plus	Alicyclobacillus spp. DNA-extraction from e.g. juices, concentrates and tomato products without preenrichment. Lee más	50 preparations	Q705
QuickGEN PCR Kit Zygosaccharomyces rouxii	Detection of Zygosaccharomyces rouxii in wine and grape must by real-time PCR. Lee más	48 reactions	Q583
SureFast® Enterobacteriaceae 4plex	The SureFast® Enterobacteriaceae 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacteriaceae, Cronobacter spp. and Salmonella spp.. Lee más	100 reactions	F5180
SureFast® Fecal Screen 4plex	The SureFast® Fecal Screen 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacteriaceae, Enterococci, Escherichia coli in water. Lee más	100 reactions	F5504
QuickGEN PCR Kit Yeast universal -low-	Qualitative detection of yeast contaminations in beverages. For exapmple: Saccharomyces spp. Dekkera spp. Zygosaccharomyces spp. Rhodotorula spp. Torulaspora spp. Kluyveromyces spp. Debaromyces spp. Candida spp. Hanseniaspora spp. Pichia spp Lee más	48 reactions: 48 x Dye Strips (freeze-dried, incl. IC-DNA)	Q982
GEN-IAL® QuickGEN P1 and Dekkera spp. Screening	Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera/ Pectinatus) and Dekkera spp. contaminations in filtrated beer, beer mixes and yeast containing samples: Lee más	48 reactions	Q091, Q092, Q093, Q094
SureFast® Listeria 3plex ONE	The SureFast® Listeria 3plex ONE is a multiplex real-time PCR test kit for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food and environmental samples. Benefits: MicroVal & … Lee más	100 reactions	F5217
QuickGEN PCR Kit Fusarium spp.	Detection of Fusarium spp. in beverages, cereals etc. by real-time PCR. Lee más	48 reactions	Q961
SureFast® Campylobacter 4plex	The SureFast® Campylobacter 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Campylobacter jejuni, Campylobacter lari and Campylobacter coli. Lee más	100 reactions	F5170
SureFast® Mag PREP Pathogens	The kit is intended to be used for the isolation of DNA and RNA viruses nucleic acids from food and feed (e.g. wash up fluids from fruits, salads etc.), filters from water samples and swabs (e.g. from work surfaces etc.) as well as bacterial DNA from … Lee más	96 preparations	F1062
SureFast® STEC 4plex ONE	The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli … Lee más	100 reactions	F5265
SureFast® MRSA 4plex	The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of … Lee más	100 reactions	F7117
TANBead Maelstrom™ 4800	The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más	Up to 48 samples	ZMAL48
SureFast® Mag PREP Food	The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más	96 preparations	F1060
GEN-IAL® QuickGEN PCR Kit Alicyclobacillus Differentiation high	Qualitative detection and differentiation of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris . Lee más	48 reactions	Q721
SureFast® Yersinia 3plex	The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica. Lee más	100 reactions	F5132
GEN-IAL® Dekkera bruxellensis Standard DNA	Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR. Lee más	1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried)	Q360
GEN-IAL® QuickGEN PCR Kit S. cerevisiae var. diastaticus white	Detection of S. cerevisiae var. diastaticus in beverages. Lee más	48 reactions	Q183
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white	Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Lee más	48 reactions	Q563
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low	Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR. Lee más	48 reactions (tubes incl. Lyticase and IC-DNA)	Q562
GEN-IAL® QuickGEN Yeast Dekkera spp.	Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus. Lee más	50 reactions	Q555
GEN-IAL® QuickGEN Yeast Dekkera spp. white	Detection of Dekkera spp. in wine and grape must by real-time PCR. Lee más	48 reactions	Q553
GEN-IAL® QuickGEN Yeast Dekkera spp. low	Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus Lee más	48 reactions	Q552
GEN-IAL® QuickGEN Yeast Dekkera spp. high	Detection of Dekkera spp. in wine and grape must by real-time PCR. Lee más	48 reactions	Q551
GEN-IAL® QuickGEN Wild yeast 2 low	Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia … Lee más	48 reactions	Q532
GEN-IAL® QuickGEN Wild yeast 1	Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila … Lee más	50 reactions	Q525
GEN-IAL® QuickGEN Wine Screening	Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control Lee más	48 reactions	Q321, Q322, Q323,Q324
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high	Detection of Saccharomyces diastaticus in beverages by real-time PCR. Lee más	48 reactions	Q181
GEN-IAL® QuickGEN Yeast Bottom fermented white	Detection of bottom-fermented yeast in beverages by real-time PCR. Lee más	48 reactions	Q163
GEN-IAL® QuickGEN Yeast Bottom fermented low	Detection of bottom-fermented yeast in beverages (e.g. beer, wine, grape must) by real-time PCR. Lee más	48 reactions	Q162
GEN-IAL® QuickGEN® Yeast Top fermented white	Real-time PCR-Kit for the detection of top-fermented yeast. Lee más	48 reactions	Q153
GEN-IAL® QuickGEN® Yeast Top fermented low	Real-time PCR-Kit for the detection of top-fermented yeast. Lee más	48 reactions	Q152
GEN-IAL® QuickGEN Beer differentiation	Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. … Lee más	The kit contains sufficient reagents for 96 PCR reactions (12 samples) 12 strips (tube 2 contains lyticase)	Q081, Q082. Q083
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation	Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild … Lee más	96 PCR reactions (24 samples) 12 stripes (tubes 2, 3, 5 and 6 contain lyticase)	Q071, Q072. Q073
GEN-IAL® QuickGEN P1 Screening and Hop resistance	Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Lee más	48 reactions	Q051, Q052, Q053, Q054, Q055
GEN-IAL® QuickGEN P1 Screening without yeast	Qualitative detection of bacterial contaminations in beer and mixed beer drinks. Lee más	48 reactions	Q031, Q032, Q033, Q034,
SureFast® Escherichia coli Serotype II 4plex	The SureFast® Escherichia coli Serotype II 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific wzx gene DNA sequences of Escherichia coli O45, wbdl gene DNA sequences of Escherichia coli O111 and … Lee más	100 reactions	F5168
SureFast® Escherichia coli Serotype I 4plex	The SureFast® Escherichia coli Serotype I 4plex is a multiplex real-time PCR kit for the direct, qualitative detection and differentiation of specfifc wzx gene DNA sequences of Escherichia coli O121, O26 and O103. Lee más	100 reactions	F5167
GEN-IAL® QuickGEN Wine Screening without yeast	Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Lee más	48 reactions	Q331, Q332, Q333, Q334
SureFast® Foodborne Pathogens 4plex	The SureFast® Foodborne Pathogens 4plex is a multiplex real-time PCR for for the direct, qualitative detection and differentiation of Escherichia coli virulence factors (stx1 [subtype a-d], stx2 [subtype a-g]), Listeria monocytogenes and Salmonella … Lee más	100 reactions	F5175
GEN-IAL® QuickGEN Wild yeast 2	Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia … Lee más	50 reactions	Q535
GEN-IAL® QuickGEN Wild yeast 1 low	Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila … Lee más	48 reactions	Q522
SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex	The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium. Each reaction contains an … Lee más	100 reactions	F5166
GEN-IAL® QuickGEN P1 Screening	Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera / Pectinatus) and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find … Lee más	48 reactions	Q021, Q022, Q023, Q024, Q025
GEN-IAL® QuickGEN Enterobacteriacea spp.	Qualitative real.time PCR-detection of Enterobacteriaceae as indicators for hygiene and postprocessing contaminations. The system contains an integrated inhibition control. Lee más	50 reactions	Q143, Q145
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus low	Detection of Saccharomyces diastaticus in beverages by real-time PCR. Lee más	48 reactions	Q182

Organismos modificados genéticamente

Soluciones innovadoras para la detección de GMO en los alimentos

Producto	Descripción	No. of tests/amount	Art. No.
TANBead Maelstrom™ 4800	The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más	Up to 48 samples	ZMAL48
SureFast® Mag PREP Food	The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más	96 preparations	F1060

PCR en tiempo real
Diagnóstico molecular para análisis alimentario