Beer analysis

Detection of spoilage organisms in beer

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Beer analysis

Do you want to test beer for beverage spoilers? For fast and specific screening and detection of microbiological spoilage parameters in breweries, a wide product line of qPCR kits is offered.

Spoilage bacteria and yeasts can cause turbidity, change of taste and off-flavors in beers. To avoid losses or recalls, it is important to test for spoilage organisms in all areas of production. For fast and specific screening and detection of microbiological spoilage parameters in beer production, we offer a wide product line of test kits. The following spoilage bacteria and yeasts are of special interest in the beer industry and should be monitored: 

Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) is a spoilage yeast in beverages, especially important for the wine industry, where it has been shown to produce high amounts of volatile phenols (4-ethylphenol and 4-ethylguaiacol) imparting off-flavors to red wines. The yeasts of the species Dekkera bruxellensis are responsible for serious economic losses in the wine industry owing to their ability to spoil wines by the production of ethylphenols. Some authorities consider “brett” to be responsible for 90% of the spoilage problems in premium red wines. However, in the production of beer (especially craft beer or Belgian beer specialities), Dekkera bruxellensis is often not considered as contaminant, but as flavor enrichment.

Lactobacillus may be part of a regular fermentation process, but can also cause undesired acidification of beer or spoilage of fruit juices. Lactobacilli are killed by standard pasteurization.

Megasphaera are gram-negative, strictly anaerobic bacteria. They are well-known as beer spoilers producing off-flavors and turbidity.

Apart from MegaspaeraPectinatus is another important spoilage bacterium in beer production. The gram-negative and strictly anaerobic bacterium causes turbidity and off-flavors reminiscent of rotten eggs.

Pediococcus is a genus of gram-positive lactic acid bacteria which are considered as contaminant in wine and beer. However, in some beer styles, the presence of Pediococcus may be desired.

Pichia is a genus of yeasts in the family Saccharomycetaceae. It is used in the fermentation process in winemaking, but it can spoil wine when present in high quantities. Pichia may also cause turbidity and off-flavors in beer.

Saccharomyces is a genus of fungi including various species of yeasts. One of the best known species is Saccharomyces cerevisiae which is used in the production of beer, wine or bread. The species Saccharomyces diastaticus is a spoilage yeast in the wine and beer industry.

Beer analysis with GEN-IAL® can help you to avoid product recalls, to minimize production losses and to prevent damage to brand image. We offer test systems for in-process control and/or for on-line final product control. Use different analytical modules to establish your customized monitoring of microbiological beer spoilage organisms in beer and beer products: Either extremely fast or maximal sensitive detection and specific identification is possible by combining different methods of sample preparation and detection with real-time PCR.

Rapid beer screening using QuickGEN & MyGo Pro

In this video, we show you how to use the real-time PCR cycler “MyGo Pro” for beer analysis. This is a very fast and comfortable way to test beer for various beer spoilage bacteria.

Our GEN-IAL® portfolio includes screening kits and beer-specific kits. The new GEN-IAL® QuickGEN line is characterized by a simple and rapid sampling and assay process, providing results within two hours after sampling. The test is based on filtration or centrifugation with subsequent qPCR and allows an extreme rapid and specific screening and detection in beer without pre-enrichment (reduced sensitivity, no differentiation in vitality). Analysis is very easy to perform even for untrained users.

The established qPCR kits based on pre-enrichment of the sample allow a sensitive screening and specific detection. The new concept of pre-coated 8-well qPCR strips (TPBD, TPYB) enables the user to screen and identify safely over 30 beer spoilage bacteria and yeasts in one qPCR run without misinterpretation and can be performed on common multiplex blockcyclers.

GEN-IAL® product overview

You can find more information on our real-time PCR test kits for beer analysis in our free brochure.Download

Test kits for beer analysis:

ProductDescriptionTubeArt. No.
GEN-IAL® QuickGEN P1 ScreeningQualitative detection of bacterial and yeast contaminationshighQ021
GEN-IAL® QuickGEN P1 Screening without yeastQualitative detection of bacterial contaminationshighQ031
GEN-IAL® QuickGEN P1 and S. diastaticus ScreeningQualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and beer mixes.highQ041
GEN-IAL® QuickGEN P1 Screening and Hop resistanceDNA screening and differentiation of beer spoiling bacteria and hop resistance geneshighQ051
GEN-IAL® QuickGEN P1 Screening and Hop resistanceDNA screening and differentiation of beer spoiling bacteria and hop resistance genesQ055
GEN-IAL® QuickGEN Beer yeast and bacteria differentiationMultiplex detection and identification of beverage spoiling bacteria and yeastshighQ071
GEN-IAL® QuickGEN Beer Differentiation PCR KitMultiplex detection (30 species) and identification (19 species) of relevant beer spoilers (bacteria and yeasts)highQ081
GEN-IAL® QuickGEN BiofilmSpecific DNA detection of Lactococcus lactis, Leuconostoc mesenteroides and Pichia anomalaQ095
ProductDescriptionArt. No.
GEN-IAL® QuickGEN Hop resistanceSpecific DNA detection of hop resistance genesQ105
GEN-IAL® QuickGEN Pectinatus/Megasphaera differentiation lowSpecific detection and differentiation of Pectinatus and MegasphaeraQ112
GEN-IAL® QuickGEN Enterobacteriaceae spp.Multiplex PCR-detection of enterobacteriaceae as indicators for hygiene and postprocessing contaminationsQ145
ProductDescriptionTubeArt. No.
GEN-IAL® QuickGEN Yeast Top fermentedSpecific DNA detection of top fermented yeasthighQ151
GEN-IAL® QuickGEN Yeast Bottom fermentedSpecific DNA detection of bottom fermented yeasthighQ161
GEN-IAL® QuickGEN Yeast Wickerhamomyces anomalusSpecific DNA detection of Pichia anomalaQ175
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticusDetection of Saccharomyces diastaticushighQ181
GEN-IAL® Dekkera bruxellensis quantitativeSpecific DNA detection of Dekkera bruxellensisSPQ380
Specific DNA detection of Dekkera bruxellensis (FAM/ROX)Q385
Specific DNA detection of Dekkera bruxellensis (FAM/HEX)Q395
GEN-IAL® QuickGEN Wild yeast 1 lowDifferentiation of wild yeastlowQ522
GEN-IAL® QuickGEN Wild yeast 1Differentiation of wild yeastQ525
GEN-IAL® QuickGEN Wild yeast 2 lowDifferentiation of wild yeastlowQ532
GEN-IAL® QuickGEN Wild yeast 2Differentiation of wild yeastQ535
GEN-IAL® QuickGEN Yeast DifferentiationDNA screening and differentiation of yeastshighQ541
GEN-IAL® QuickGEN Yeast Dekkera spp.Specific DNA detection of Dekkera bruxellensishighQ551
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailiiSpecific DNA detection of Zygosaccharomyces bailiihighQ561
GEN-IAL® Pichia membranaefaciensSpecific DNA detection of Pichia membranaefaciensQ930

Please note: The last digit of the article number identifies the tube version (1=high, 2=low, 3=white, 4=MyGo Pro, 5=liquid reagents without precoated strips). For example, the article number Q331 means high tubes, Q332 means low tubes, Q333 means white tubes and Q334 means low MyGo Pro tubes.

DNA preparation:

ProductDescriptionArt. No.
GEN-IAL® QuickGEN Sample Preparation CentrifugationDNA preparation of beverage samples, CentrifugationQ002
GEN-IAL® QuickGEN Sample Preparation FiltrationDNA preparation of beverage samples, FiltrationQ004
GEN-IAL® QuickGEN Yeast Sample Preparation CentrifugationMicrobial DNA-Extraction from samples with high yeast cell counts e.g. yeast tanks, propagation etc.Q005

What is the right test kit for you?

Our “decision tree” helps you to find the real-time PCR kit that best suits your needs in beer analysis.Download