Gliadin / Gluten

Test kits for gluten detection in food

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Gluten detection in food – Measurement of Gliadin

People suffering from celiac disease must avoid food containing gluten. In addition, a growing group of consumers stays away from gluten containing foods. Consumers rely on gluten-free labeling and producers have to ensure their products meet gluten-free regulations. Reliable, validated gluten test kits offer gluten-free food producers the optimal tool for gluten testing.

Producing healthy, tasty products that are gluten-free can be a challenge. Manufacturers producing gluten free food must assure their products meet legal requirements for gluten-free food. Testing for gluten (gliadin) is a key element in quality control. We offer an extensive range of test kits for quality control of your products.

Lateral flow test for gluten analysis

You can start by testing on surfaces in the production areas. Use a lateral flow test like the RIDA®QUICK Gliadin for swabbing surfaces. Bear in mind there might be also gluten traces in the laboratory. So before testing for gluten, check if your laboratory is free of gluten traces. The RIDA®QUICK Gliadin lateral flow test can also be used for testing ingredients and raw materials.

Gluten extraction methods

After you have checked your production environment for gluten traces, test your raw materials and finished products for the presence of gluten. Effective extraction of gluten from the food is a crucial step in analysis. Depending on the food matrix and test method selected, you can choose an ethanol extraction or use the patented Mendez cocktail extraction solution.

Use ethanol extraction in combination with the RIDASCREEN® Gliadin competitive or RIDA®QUICK Gliadin. Use the cocktail solution in combination with the RIDASCREEN® Gliadin, RIDASCREEN®FAST Gliadin or RIDA®QUICK Gliadin:

ELISA test for gluten analysis

Finally choose the appropriate gluten test kit for your products. Use the sandwich ELISA for testing most food products, use the competitive ELISA for testing fermented or hydrolyzed products like beer, syrups or sourdough.

All mentioned test kits are based on the R5 antibody (Mendez). Codex Alimentarius specifies in Codex Standard 118-1979 (2008) the R5 antibody based ELISA as the standard method for the determination of gluten. The RIDASCREEN® Gliadin ELISA and RIDASCREEN® Gliadin competitive are AOAC Official Methods of Analysis.

PCR test for gluten analysis

PCR is versatile as it can be used to confirm ELISA results, and it can be used qualitatively or quantitatively. All qPCR kits have a standardized sample and test procedure. Moreover, semi-automated sample preparation is available. 

The unique SureFood® ALLERGEN 4plex Cereals (S7006) kit differentiates the cereal species wheat, barley and rye.

Product overview

You can find a comprehensive overview of our gluten test kits in our free brochure.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
EuroProxima Gluten-Tec®

EuroProxima Gluten-Tec® is a competitive enzyme immunoassay for the quantitative determination of gliadin and gliadin fragments in food.

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Microtiter plate with 96 wells (12 strips with 8 wells each). 5171GT
RIDASCREEN® Total Gluten

RIDASCREEN® Total Gluten (Art. No. R7041) is a sandwich enzyme immunoassay for the quantitative determination of intact (non-hydrolyzed) gluten from gluten containing cereals (wheat, rye and barley) in oat and oat products.

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7041
RIDASCREEN®FAST Gliadin sensitive

RIDASCREEN®FAST Gliadin sensitive is a sandwich enzyme immunoassay for the determination of prolamins from gluten containing cereals in as gluten-free declared food (cookies, rice flour, oat flour, sausages, quinoa flour, chocolate, millet flour).

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7051
RIDASCREEN® Gliadin

RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food. It is applicable for the quantitative …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7001
RIDASCREEN® Gliadin competitive

RIDASCREEN® Gliadin competitive is used for the analysis of fermented and hydrolyzed food (e.g. beer, starch syrup, starch, malt extract, sourdough, soy sauce) which are declared as “gluten-free”. The R5 monoclonal antibody recognizes …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7021
RIDASCREEN®FAST Gliadin

RIDASCREEN® FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R7002
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ProductDescriptionNo. of tests/amountArt. No.
RIDA®QUICK Gliadin

RIDA®QUICK Gliadin can be used as a swab test for gluten detection on surfaces in the hygiene control, in cleansing waters, and for the qualitative detection of gliadin / gluten in raw material and processed food. The test has been developed for the …

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25 x test strips in reclosable tube
for 25 determinations
R7003
RIDA®QUICK Gliadin (single packaged)

RIDA®QUICK Gliadin (single packaged) can be used for gluten detection on surfaces for hygiene control (swab test), in cleansing waters, and for the qualitative detection of gliadin / gluten in raw material and processed food. The test has been …

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25 x test strips (single packaged)
for 25 determinations
R7004
RIDA®QUICK Gliadin (ready to swab)

RIDA®QUICK Gliadin (ready to swab) can be used as a swab test for the gluten detection on surfaces in the hygiene control and in cleansing waters (CIP water). The test has been developed for the detection of low amounts of gluten (contamination). No …

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25 x test strips (single packaged),
25 prefilled vials with ready-to-use sample buffer,
for 25 determinations
R7005
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ProductDescriptionNo. of tests/amountArt. No.
SureFood® ALLERGEN 4plex Cereals

The SureFood® ALLERGEN 4plex Cereals is a real-time PCR test for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences in food. Each reaction contains an …

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100 reactions S7006
SureFood® ALLERGEN Gluten

The SureFood® ALLERGEN Gluten is a real-time PCR for the direct, qualitative and / or quantitative detection of specific gluten-containing cereals (wheat such as spelt and khorasan wheat, rye, barley, oats) DNA sequences according to directive (EC) …

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100 reactions S3606
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