Spoilage organisms
Detect microbiological spoilage in food production

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Analytes

Spoilage organisms in food production

Importance of monitoring spoilage organisms

In addition to detecting pathogenic microorganisms, monitoring typical spoilage organisms is crucial to reduce losses during production. Spoilage organisms, while not toxic, can alter the taste, smell, appearance, and shelf life of food products, leading to significant economic losses. Testing for spoilage organisms in all areas of production helps prevent these issues and avoid product recalls.

Potential contamination in various commodities

All types of commodities are susceptible to contamination by spoilage organisms and pathogens. Spoilage organisms can affect a wide range of products, including:

  • Meat and meat products
  • Dairy products
  • Eggs and egg products
  • Vegetables, fruits, herbs, and spices
  • Beverages
  • Cereals and cereal products
  • Prepared meals

Testing methods

To effectively manage spoilage, R-Biopharm offers reliable real-time PCR kits for analyzing various products. Real-time PCR provides highly specific detection of spoilage organisms using advanced molecular techniques, ensuring accurate and rapid results.

Beverage spoilage

Spoilage organisms are a significant problem in the beverage industry. Spoilage bacteria and yeasts can cause turbidity and off-flavors in beverages. For fast and specific screening and detection of microbiological spoilage parameters in beverages, R-Biopharm offers a comprehensive product line of qPCR kits.

Key benefits of monitoring spoilage organisms

  • Quality control: Ensures the taste, smell, appearance, and shelf life of food products are maintained.
  • Economic savings: Reduces losses and avoids costly product recalls.
  • Comprehensive testing: Provides reliable detection across various food commodities.
  • Advanced technology: Utilizes cutting-edge real-time PCR methods for accurate results.

Conclusion

Monitoring spoilage organisms is essential for maintaining the quality and economic viability of food products. R-Biopharm’s range of testing kits provides reliable and effective solutions for detecting spoilage organisms across a wide range of commodities. Implementing these testing methods helps ensure product quality and prevent economic losses due to spoilage.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
QuickGEN PCR Kit Screening and differentiation of wine spoilers

Screening and differentiation of 8 bacteria and yeast in wine. The following bacteria and yeasts are differentiated: Lactobacilli / Pediococci, Oenococcus oeni, Acetic acid bacteria, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Dekkera …

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96 reactions / 24 samples Q231, Q232, Q233
QuickGEN PCR Kit Screening and differentiation of wine spoilers

Screening and differentiation of 14 bacteria and yeast in wine. The following yeast are differentiated: Lactobacillus spp. Pediococcus spp. Acetic acid bacteria Leuconostoc mesenteroides Oenococcus oeni Lactococcus lactis Alicyclobacillus spp. …

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12 samples / 96 reatcions Q221, Q222, Q223
Simplex® Easy Spin Plus

Alicyclobacillus spp. DNA-extraction from e.g. juices, concentrates and tomato products without preenrichment.

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50 preparations Q705
QuickGEN PCR Kit Zygosaccharomyces rouxii

Detection of Zygosaccharomyces rouxii in wine and grape must by real-time PCR.

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48 reactions Q583
QuickGEN PCR Kit Yeast universal -low-

Qualitative detection of yeast contaminations in beverages. For exapmple: Saccharomyces spp. Dekkera spp. Zygosaccharomyces spp. Rhodotorula spp. Torulaspora spp. Kluyveromyces spp. Debaromyces spp. Candida spp. Hanseniaspora spp. Pichia spp

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48 reactions: 48 x Dye Strips (freeze-dried, incl. IC-DNA) Q982
GEN-IAL® QuickGEN P1 and Dekkera spp. Screening low MG

Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera/ Pectinatus) and Dekkera spp. contaminations in filtrated beer, beer mixes and yeast containing samples:  

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48 reactions Q094
QuickGEN Pentaplex PCR Kit – Beer spoilage bacteria and yeast screening

Qualitative detection of bacterial, S. cerevisiae var. diastaticus and Dekkera spp. contaminations in filtered beer, beer mixes and yeast containing samples.

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48 reactions Q061
GEN-IAL® QuickGEN PCR Kit Alicyclobacillus Differentiation high

Qualitative detection and differentiation of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris .

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48 reactions Q721
GEN-IAL® Dekkera bruxellensis Standard DNA

Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR.

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1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried) Q360
GEN-IAL® QuickGEN PCR Kit S. cerevisiae var. diastaticus white

Detection of S. cerevisiae var. diastaticus in beverages.

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48 reactions Q183
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions Q563
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions (tubes incl. Lyticase and IC-DNA) Q562
GEN-IAL® QuickGEN Yeast Dekkera spp.

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus.

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50 reactions Q555
GEN-IAL® QuickGEN Yeast Dekkera spp. white

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q553
GEN-IAL® QuickGEN Yeast Dekkera spp. low

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus

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48 reactions Q552
GEN-IAL® QuickGEN Yeast Dekkera spp. high

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q551
GEN-IAL® QuickGEN Yeast differentiation white

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples Q543
GEN-IAL® QuickGEN Yeast differentiation low

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples (tubes contain lyticase). Q542
GEN-IAL® QuickGEN Wild yeast 2 low

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia …

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48 reactions Q532
GEN-IAL® QuickGEN Wild yeast 1

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila …

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50 reactions Q525
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/HEX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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50 reactions Q395
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/ROX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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50 reactions Q385
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative white

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions Q373
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative low

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). Q372
GEN-IAL® QuickGEN Wine Screening without yeast low MG

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q334
GEN-IAL® QuickGEN Wine Screening without yeast white

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q333
GEN-IAL® QuickGEN Wine Screening without yeast low

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q332
GEN-IAL® QuickGEN Wine Screening low MG

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control

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48 reactions Q324
GEN-IAL® QuickGEN Wine Screening white

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control

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48 reactions Q323
GEN-IAL® QuickGEN Wine Screening low

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

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48 reactions Q322
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high

Detection of Saccharomyces diastaticus in beverages by real-time PCR.

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48 reactions Q181
GEN-IAL® QuickGEN Yeast Bottom fermented white

Detection of bottom-fermented yeast in beverages by real-time PCR.

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48 reactions Q163
GEN-IAL® QuickGEN Yeast Bottom fermented low

Detection of bottom-fermented yeast in beverages (e.g. beer, wine, grape must) by real-time PCR.

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48 reactions Q162
GEN-IAL® QuickGEN® Yeast Top fermented white

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q153
GEN-IAL® QuickGEN® Yeast Top fermented low

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q152
GEN-IAL® QuickGEN Beer differentiation white

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q083
GEN-IAL® QuickGEN Beer differentiation low

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q082
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation white

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

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96 PCR reactions (24 samples)
12 stripes (tubes 2, 3, 5 and 6 contain lyticase)
Q073
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation low

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

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96 PCR reactions (24 samples)
12 stripes
Q072
GEN-IAL® QuickGEN P1 Screening and Hop resistance

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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50 reactions Q055
GEN-IAL® QuickGEN P1 Screening and Hop resistance low MG

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q054
GEN-IAL® QuickGEN P1 Screening and Hop resistance white

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q053
GEN-IAL® QuickGEN P1 Screening and Hop resistance low

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q052
GEN-IAL® QuickGEN P1 and S. diastaticus Screening

Qualitative detection of bacterial and S. cerevisiae var. diastaticus contaminations in filtrated beer, beer mixes and yeast containing samples.

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50 reactions Q045
GEN-IAL® QuickGEN P1 and S. diastaticus Screening low MG

Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera/ Pectinatus) and S. diastaticus contaminations in filtrated beer and mixed beer drinks and yeast containing samples.

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48 reactions Q044
GEN-IAL® QuickGEN P1 and S. diastaticus Screening white

Qualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and mixed beer drinks.

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48 reactions Q043
GEN-IAL® QuickGEN P1 and S. diastaticus Screening low

Qualitative detection of bacterial and S. cerevisiae var. diastaticus contaminations in filtrated beer, beer mixes and yeast containing samples.

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48 reactions Q042
GEN-IAL® QuickGEN P1 Screening without yeast

Qualitative detection of bacterial contaminations in beer and mixed beer drinks.

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50 reactions Q035
GEN-IAL® QuickGEN P1 Screening without yeast white

Qualitative detection of bacterial contaminations in beer and mixed beer drinks.

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48 reactions Q033
GEN-IAL® QuickGEN P1 Screening without yeast low

Qualitative detection of bacterial contaminations in beer and mixed beer drinks.

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48 reactions Q032
GEN-IAL® QuickGEN P1 Screening

Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find bacterial contaminations more sensitive (Q035).

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50 reactions Q025
GEN-IAL® QuickGEN P1 Screening low MG

Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera/ Pectinatus)  and yeast contaminations in filtrated beer and beer mixes.

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48 reactions Q024
GEN-IAL® QuickGEN P1 Screening white

Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera/ Pectinatus) and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find …

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48 reactions Q023
GEN-IAL® QuickGEN P1 Screening low

Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find bacterial contaminations more sensitive (Q032).

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48 reactions Q022
GEN-IAL® QuickGEN Wine Screening without yeast high

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

Read more
48 reactions Q331
GEN-IAL® QuickGEN Wine Screening high

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must: Lactobacillus/ Pediococcus/Oenococcus oeni Acetic acid bacteria Yeast Inhibition control

Read more
48 reactions Q321
GEN-IAL® QuickGEN Wild yeast 2

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia …

Read more
50 reactions Q535
GEN-IAL® QuickGEN Wild yeast 1 low

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila …

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48 reactions Q522
GEN-IAL® QuickGEN P1 Screening high

Qualitative detection of bacterial (Lactobacillus/ Pediococcus / Megasphaera / Pectinatus) and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find …

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48 reactions Q021
GEN-IAL® QuickGEN Enterobacteriacea spp.

Qualitative real.time PCR-detection of Enterobacteriaceae as indicators for hygiene and postprocessing contaminations. The system contains an integrated inhibition control.

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50 reactions Q145
GEN-IAL® QuickGEN P1 Screening without yeast high

Qualitative detection of bacterial contaminations in beer and mixed beer drinks.

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48 reactions
tube strips (freeze-dried, incl. IC-DNA)
Q031
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus low

Detection of Saccharomyces diastaticus in beverages by real-time PCR.

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48 reactions Q182
GEN-IAL® QuickGEN P1 and S. diastaticus Screening high

Qualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and mixed beer drinks and yeast containing samples.

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48 reactions
tube strips (freeze-dried, incl. IC-DNA and lyticase)
Q041
GEN-IAL® QuickGEN Yeast differentiation high

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks. The following yeast are differentiated: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii …

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96 reactions for 12 samples (tubes contain lyticase). Q541
GEN-IAL® QuickGEN P1 Screening and Hop resistance high

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q051
GEN-IAL® QuickGEN Yeast Wickerhamomyces anomalus

Detection of Wickerhamomyces anomalus in beverages (e.g. beer, wine, grape must) by real-time PCR.

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50 reactions Q175
GEN-IAL® QuickGEN® Yeast Top fermented high

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q151
GEN-IAL® QuickGEN Yeast Bottom fermented high

Detection of bottom-fermented yeast in beverages by real-time PCR.

Read more
48 reactions Q161
GEN-IAL® QuickGEN Pectinatus / Megasphaera differentiation low

Real-time PCR-Kit for the detection of Pectinatus spp. and Megasphaera spp in beverages.

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48 reactions Q112
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation high

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

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96 PCR reactions (24 samples)
12 stripes
Q071
GEN-IAL® QuickGEN Hop resistance

Qualitative detection and differentiation of the hop resistance genes hor A / hor C / hit A and ORF 5 as genetic markers for beer-spoilage microorganisms.

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50 reactions Q105
GEN-IAL® QuickGEN Beer differentiation high

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q081
GEN-IAL® Biogenic amines

Real-time PCR kit for the detection of biogenic amines producing lactic acid bacteria in wine.

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50 reactions Q345
GEN-IAL® QuickGEN Biofilm

Real-time PCR detection of Leuconostoc mesenteroides, Lactoccocus lactis and Wickerhamomyces anomalus, which are involved in the development of biofilms.

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50 reactions Q095
GEN-IAL® QuickGEN Alicyclobacillus differentiation

Qualitative detection and identification of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris.

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48 reactions Q724
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative high

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). Q371
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii high

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions (tubes incl. Lyticase and IC-DNA) Q561
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