Genetically modified organisms
Innovative solutions for the detection of GMO in food
Genetically modified organisms
Genetically modified plants (GMOs) are mainly soy, maize, rapeseed and cotton (not used as food).
Labelling of GMO-free food.
As stated by the Council of Ministers of the European Union (EU), regulation for the labelling of Novel Food: Decision No. 1139/98/EC, all food products which are containing genetically modified organisms (GMO) must be labelled. Furthermore, the EU directive 1829/2003 determines the labeling threshold value at 0.9 % in EU for food and feed.
GMO analysis according to VLOG
Further information about the VLOG requirements and the recommended analytics can be found in our flyer.
GMO analysis according to VLOG
Further information about the VLOG requirements and the recommended analytics can be found in our flyer.
Qualitative GMO detection
According to national or regional regulations, zero tolerance applies to non-approved GMO events. As such, only qualitative tests are of interest for these products at the moment. The detection limit lies at 0.01 %, depending on the matrix and the application.
Regulation EC 619/2011 exists as a special rule for animal feeds. Provided that certain conditions are met (expired EU approval, approval in the country of origin, availability of tests and reference material, etc.), a technical threshold value of 0.1 % applies for non-approved GMO events. This is particularly significant for not always contamination-free imports of GMOs and GMO-free maize and soya.
Quantitative GMO detection
For respectively approved GMO events, there is the quantitative question of whether the product lies above the defined threshold values or mandatory labelling values.
In Europe, a value of 0.9 % applies to the GMO event in the respective plant matrix. Below that value, foodstuffs with technically unavoidable and coincidental contaminations of the GMO events do not need to be declared (EC 2829/2003 und EC2830/EC). The quantification occurs via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.
GMO screening
A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides efficient evidence of the presence of any possible GMOs.
The typically used gene sequences are:
The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049).
SureFood® GMO PLANT PLUS, Art. No. S2049
SureFood® GMO PLANT 4plex Corn/Soya/Canola/Cotton, Art. No. S2156
SureFood® GMO PLANT 4plex Corn/Soya/Canola + IAC, Art. No. S2158
A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides evidence of the presence of any possible GMOs. The typically used gene sequences are 35S, NOS, FMV, BAR and CTP2:CTP4 EPSPS. These and further parameters can either be combined in a modular way or used individually.
SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC, Art. No. S2126
SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS, Art No. S2127
SureFood® GMO SCREEN 4plex BAR/PAT/CrylAb/IAc/CTP2:CP4 EPSPS, Art. No. S2128
SureFood® GMO SCREEN P35S:BAR Rice, Art. No. S2022
The multiplex approach as well as the standardized PCR set-up and analysis allow a streamlined and efficient process.
Soya:
SureFood® GMO ID 4plex Soya I, Art. No. S2161
SureFood® GMO ID 4plex Soya II, Art. No. S2162
Corn:
SureFood® GMO ID 4plex Corn I, Art. No. S2170
Canola:
SureFood® GMO ID 4plex Canola I, Art. No. S2166
SureFood® GMO ID 4plex Canola II, Art. No. S2167
Quantification is carried via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.
Soya:
SureFood® GMO QUANT RR2Y Soya, Art. No. S2029
SureFood® GMO QUANT 35S Soya, Art. No. S2028
SureFood® GMO QUANT RoundUp Ready Soya, Art. No. S2014
Corn:
SureFood® GMO QUANT Bt176 Corn, Art. No. S2015
SureFood® GMO QUANT Bt11 Corn, Art. No. S2016
SureFood® GMO QUANT Mon810 Corn, Art. No. S2019
SureFood® GMO QUANT MON863 Corn, Art. No. S2051
SureFood® GMO QUANT MIR162 Corn, Art. No. S2135
SureFood® GMO QUANT T25 Corn, Art. No. S2017
SureFood® GMO QUANT 35S Corn, Art. No. S2020
These and further parameters can either be combined in a modular way or used individually. The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049).
SureFood® is a modular system which is based on the 3 main components , amplification and detection. Each component consists of different modules. All modules are compatible. Thus, SureFood® is a highly flexible system which can be easily configured for different user specific applications. SureFood® is available as product and service as well.
SureFood® GMO product overview
You can find a comprehensive overview of our SureFood® GMO test kits in our free brochure.
