Genetically modified organisms
Innovative solutions for the detection of GMO in food
Genetically modified organisms
Genetically modified plants (GMOs) are mainly soy, maize, rapeseed and cotton (not used as food).
Labelling of GMO-free food.
As stated by the Council of Ministers of the European Union (EU), regulation for the labelling of Novel Food: Decision No. 1139/98/EC, all food products which are containing genetically modified organisms (GMO) must be labelled. Furthermore, the EU directive 1829/2003 determines the labeling threshold value at 0.9 % in EU for food and feed.
GMO analysis according to VLOG
Further information about the VLOG requirements and the recommended analytics can be found in our flyer.
GMO analysis according to VLOG
Further information about the VLOG requirements and the recommended analytics can be found in our flyer.
Qualitative GMO detection
According to national or regional regulations, zero tolerance applies to non-approved GMO events. As such, only qualitative tests are of interest for these products at the moment. The detection limit lies at 0.01 %, depending on the matrix and the application.
Regulation EC 619/2011 exists as a special rule for animal feeds. Provided that certain conditions are met (expired EU approval, approval in the country of origin, availability of tests and reference material, etc.), a technical threshold value of 0.1 % applies for non-approved GMO events. This is particularly significant for not always contamination-free imports of GMOs and GMO-free maize and soya.
Quantitative GMO detection
For respectively approved GMO events, there is the quantitative question of whether the product lies above the defined threshold values or mandatory labelling values.
In Europe, a value of 0.9 % applies to the GMO event in the respective plant matrix. Below that value, foodstuffs with technically unavoidable and coincidental contaminations of the GMO events do not need to be declared (EC 2829/2003 und EC2830/EC). The quantification occurs via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.
GMO screening
A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides efficient evidence of the presence of any possible GMOs.
The typically used gene sequences are:
The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049).
SureFood® GMO PLANT PLUS, Art. No. S2049
SureFood® GMO PLANT 4plex Corn/Soya/Canola/Cotton, Art. No. S2156
SureFood® GMO PLANT 4plex Corn/Soya/Canola + IAC, Art. No. S2158
A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides evidence of the presence of any possible GMOs. The typically used gene sequences are 35S, NOS, FMV, BAR and CTP2:CTP4 EPSPS. These and further parameters can either be combined in a modular way or used individually.
SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC, Art. No. S2126
SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS, Art No. S2127
SureFood® GMO SCREEN 4plex BAR/PAT/CrylAb/IAc/CTP2:CP4 EPSPS, Art. No. S2128
SureFood® GMO SCREEN P35S:BAR Rice, Art. No. S2022
The multiplex approach as well as the standardized PCR set-up and analysis allow a streamlined and efficient process.
Soya:
SureFood® GMO ID 4plex Soya I, Art. No. S2161
SureFood® GMO ID 4plex Soya II, Art. No. S2162
Corn:
SureFood® GMO ID 4plex Corn I, Art. No. S2170
Canola:
SureFood® GMO ID 4plex Canola I, Art. No. S2166
SureFood® GMO ID 4plex Canola II, Art. No. S2167
Quantification is carried via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.
Soya:
SureFood® GMO QUANT RR2Y Soya, Art. No. S2029
SureFood® GMO QUANT 35S Soya, Art. No. S2028
SureFood® GMO QUANT RoundUp Ready Soya, Art. No. S2014
Corn:
SureFood® GMO QUANT Bt176 Corn, Art. No. S2015
SureFood® GMO QUANT Bt11 Corn, Art. No. S2016
SureFood® GMO QUANT Mon810 Corn, Art. No. S2019
SureFood® GMO QUANT MON863 Corn, Art. No. S2051
SureFood® GMO QUANT MIR162 Corn, Art. No. S2135
SureFood® GMO QUANT T25 Corn, Art. No. S2017
SureFood® GMO QUANT 35S Corn, Art. No. S2020
These and further parameters can either be combined in a modular way or used individually. The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049).
SureFood® is a modular system which is based on the 3 main components , amplification and detection. Each component consists of different modules. All modules are compatible. Thus, SureFood® is a highly flexible system which can be easily configured for different user specific applications. SureFood® is available as product and service as well.
SureFood® GMO product overview
You can find a comprehensive overview of our SureFood® GMO test kits in our free brochure.
Product portfolio
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Read more |
Up to 48 samples | ZMAL48 |
SureFood® GMO QUANT MON810 Corn |
The test detects the relative quantitative MON810 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-ØØ81Ø-6) and the other one specific for Corn (the reference gene). The … Read more |
2 x 50 reactions | S2019 |
SureFood® GMO ID DAS-40278-9 Corn |
The SureFood® GMO ID DAS-40278-9 Corn is a real-time PCR kit for the direct, qualitative detection of a specific genetically modified corn DNA sequence. Therefore the kit contains an event specific real-time PCR system for the DAS-40278-9 corn (OECD … Read more |
100 reactions | S2140 |
SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Read more |
96 preparations | F1060 |
SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS |
The SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences. Phosphinothricin-Acetyltransferase gene … Read more |
100 reactions | S2128 |
SureFood® GMO ID 4plex Corn I |
The SureFood® GMO ID 4plex Corn I is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences: FAM channel: MON810 corn (OECD unique identifier MON-ØØ81Ø-6) VIC/HEX … Read more |
100 reactions | S2170 |
SureFood® GMO ID 4plex Soya II |
SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below: Roundup Ready Soya (RR Soya) (OECD unique identifier … Read more |
100 reactions | S2162 |
SureFood® GMO ID 4plex Canola II |
The multiplex test detects the following DNA-sequences of genetically modified canola: – FAM channel: MON88302 canola (OECD unique identifier MON-883Ø2-9) – VIC channel: Canola – ROX channel: DP73496 canola (OECD unique identifier … Read more |
100 reactions | S2167 |
SureFood® GMO ID 4plex Canola I |
The multiplex test detects the following DNA sequences of genetically modified canola: FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8) ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7) Cy5 channel: T45 canola (OECD … Read more |
100 reactions | S2166 |
SureFood® GMO ID 4plex Soya I |
The multiplex test SureFood® GMO ID 4plex Soya I detects the following DNA-sequences of soya: FAM-channel: MON87708 Soja (OECD Bezeichnung MON-877Ø8-9) FAM-channel: CV127 Soja (OECD Bezeichnung BPS-CV127-9) VIC-channel: DP305423 Soja (OECD … Read more |
100 reactions | S2161 |
SureFood® GMO ID CV127 Soya (R&D Version) |
Detection of CV 127 Soya. Read more |
100 reactions | S2066 |
SureFood® GMO ID DP305423 Soya (R&D Version) |
The test detects DP305423 Soya (OECD unique identifier DP-3Ø5423-1) DNA. The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments … Read more |
100 reactions | S2065 |
SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS |
This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. The multiplex test detects the following DNA-sequences: Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius … Read more |
100 reactions | S2127 |
SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC |
The SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC is a real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola DNA sequences. Read more |
100 reactions | S2158 |
SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton |
The SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton is a real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola/cotton DNA sequences. Read more |
100 reactions | S2156 |
SureFood® GMO QUANT GT73 Canola |
SureFood® GMO QUANT GT73 Canola is a real-time PCR for the detection of the relative GT73 canola DNA amount to the total canola DNA content. Therefore, the kit contains two PCR systems, one for detection of a GT73 canola specific gene (OECD unique … Read more |
2 x 50 reactions | S2061 |
SureFood® GMO QUANT MON863 Corn |
SureFood® GMO QUANT MON863 Corn is a real-time PCR for the detection of the relative MON863 corn DNA amount to the total corn DNA content. Therefore, the kit contains two PCR systems, one for detection of a MON863 corn-specific gene (YieldGard™ … Read more |
2 x 50 reactions | S2051 |
SureFood® GMO PLANT PLUS |
The test detects DNA of plants qualitatively. Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S2049 |
SureFood® GMO QUANT MIR162 Corn |
The test detects the relative quantitative MIR162-Corn DNA amount. The kit contains two PCR systems, one event-specific for the MIR162-Corn (OECD unique identifier SYN-IR162-4) and the other one specific for Corn (reference system). Read more |
2 x 50 reactions | S2135 |
SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC |
This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S Cauliflower Mosaic Virus (CaMV) promoter DNA sequence, A. tumefaciens NOS terminator DNA … Read more |
100 reactions | S2126 |
SureFood® GMO SCREEN CaMV |
The test detects CaMV DNA. The presence of CaMV DNA indicates an infestation with the cauliflower mosaic virus. Host of the CaMV are Brassicaceae plants. With the virus specific detection system SureFood® GMO CaMV it is possible to verify the … Read more |
100 reactions | S2027 |
SureFood® GMO QUANT T25 Corn |
The test detects the relative quantitative T25-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the T25-Corn (OECD unique identifier ACS-ZMØØ3-2) and the other one specific for Corn (reference system). The … Read more |
2 x 50 reactions | S2017 |
SureFood® GMO QUANT RR2Y Soya |
SureFood® GMO QUANT RR2Y Soya is a real-time PCR for the detection of the relative Roundup Ready 2 Yield® Soya DNA amount (RR2Y soya) to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR2Y … Read more |
2×50 reactions | S2029 |
SureFood® GMO QUANT RoundUp Ready Soya |
SureFood® GMO QUANT Roundup Ready Soya (RR soya) is a real-time PCR for the detection of the relative RR soya DNA amount to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR soya specific gene … Read more |
2×50 reactions | S2014 |
SureFood® GMO QUANT Bt176 Corn |
The test detects the relative quantitative Bt176-Corn DNA amount. Therefore the kit contains two PCR systems, one construct specific for the Bt176-Corn (OECD unique identifier SYN-EV176-9) and the other one specific for Corn (reference system). … Read more |
2 x 50 reactions | S2015 |
SureFood® GMO QUANT Bt11 Corn |
The test detects the relative quantitative Bt11 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the event Bt11 Corn (OECD unique identifier SYN-BTØ11-1) and the other one specific for Corn (the reference gene). The … Read more |
2 x 50 reactions | S2016 |
SureFood® GMO QUANT 35S Soya |
SureFood® GMO QUANT 35S Soya is a real-time PCR for the detection of the relative 35S CaMV promoter DNA amount to the total soya DNA content in food, feed and seeds. Therefore, the kit contains two PCR systems, one for detection of a 35S CaMV … Read more |
2×50 reactions | S2028 |
SureFood® GMO QUANT 35S Corn |
The test is developed for the relative estimation of the 35S CaMV DNA amount in only Corn containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Corn (the … Read more |
2×50 reactions, whereof 50 reactions are used for the quantification of the reference gene. | S2020 |
SureFood® GMO SCREEN P35S:BAR Rice |
The SureFood® GMO SCREEN P35S:BAR Rice is a real-time PCR for the direct, qualitative detection of a specific P35S:BAR constructDNA sequence of P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OSØØ3-7) Rice and the … Read more |
100 reactions, whereas 50 reactions are used for detection of the reference gene. | S2022 |
SureFood® GMO ID Bt63 Rice |
The SureFood® GMO ID Bt63 Rice is a real-time PCR for the direct, qualitative detection of a specific genetically modified Bt63 rice DNA sequence. Therefore, the kit contains a construct specific real-time PCR system for the Bt63 rice (OECD unique … Read more |
2 x 50 reactions | S2024 |