Genetically modified organisms
Innovative solutions for the detection of GMO in food
Genetically modified organisms
The world wide production of genetically modified food (e.g. soy and corn) has steadily increased since the beginning of the 90’s.
Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labelled. As stated by the Council of Ministers of the European Union (EU), regulation for the labelling of Novel Food: Decision No. 1139/98/EC. Furthermore, since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0.9 % in EU for food and feed.
Labelling of GMO-free food
The quality of food is becoming increasingly important to many consumers. To ensure the necessary transparency, i.a. the European Council Regulation (EC) (No. 834/2007) on organic production was implemented. This regulation regulates the use and labeling of genetically modified plants in food and feed (European labeling requirements 1829/2003 and 1830/2003). Compliance with this basic organic regulation can be shown by a corresponding seal on the food products.
GMO analysis according to VLOG
Further information about the VLOG requirements and the recommended analytics can be found in our flyer.
Qualitative GMO detection
According to national or regional regulations, zero tolerance applies to non-approved GMO events. As such, only qualitative tests are of interest for these products at the moment. The detection limit lies at 0.01 %, depending on the matrix and the application.
Regulation EC 619/2011 exists as a special rule for animal feeds. Provided that certain conditions are met (expired EU approval, approval in the country of origin, availability of tests and reference material, etc.), a technical threshold value of 0.1 % applies for non-approved GMO events. This is particularly significant for not always contamination-free imports of GMOs and GMO-free maize and soya.
Quantitative GMO detection
For respectively approved GMO events, there is the quantitative question of whether the product lies above the defined threshold values or mandatory labelling values.
In Europe, a value of 0.9 % applies to the GMO event in the respective plant matrix. Below that value, foodstuffs with technically unavoidable and coincidental contaminations of the GMO events do not need to be declared (EC 2829/2003 und EC2830/EC). The quantification occurs via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.
GMO screening
A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides efficient evidence of the presence of any possible GMOs.
The typically used gene sequences are:
This promoter, made from the cauliflower mosaic virus, is the most frequently used element. Plant samples that are only 35S positive can also be contaminated with the naturally occurring virus. False positive results can be ruled out by using the virus-specific CaMV (S2027) test.
The Nopalin Synthase Terminator (NOS) from Agrobacterium tumefaciens is often but not exclusively used in combination with 35S.
In lieu of 35S, the figwort mosaic virus promoter can also be used in GMOs.
The gene for phosphinotricin-N-acetyltransferase from Streptomyces hygroscopicus can enable a resistance against the herbicide glufosinate that is available under several different brand names.
This sequence denotes the transition from chloroplast transit peptide to 5-enolpyruvylshikimate-3-phosphate synthase from Arabidopsis thaliana and Agrobacterium spp. It enables a resistance against the herbicide glyphosate.
These and further parameters can either be combined in a modular way or used individually. The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049).
SureFood® is a modular system which is based on the 3 main components , amplification and detection. Each component consists of different modules. All modules are compatible. Thus, SureFood® is a highly flexible system which can be easily configured for different user specific applications. SureFood® is available as product and service as well.
SureFood® GMO product overview
You can find a comprehensive overview of our SureFood® GMO test kits in our free brochure.
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Read more |
Up to 48 samples | ZMAL48 |
| SureFood® GMO QUANT MON810 Corn |
The test detects the relative quantitative MON810 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-ØØ81Ø-6) and the other one specific for Corn (the reference gene). The … Read more |
2 x 50 reactions | S2019 |
| SureFood® GMO ID DAS-40278-9 Corn |
The SureFood® GMO ID DAS-40278-9 Corn is a real-time PCR kit for the direct, qualitative detection of a specific genetically modified corn DNA sequence. Therefore the kit contains an event specific real-time PCR system for the DAS-40278-9 corn (OECD … Read more |
100 reactions | S2140 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of … Read more |
96 preparations | F1060 |
| SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS |
The SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences. Phosphinothricin-Acetyltransferase gene … Read more |
S2128 | |
| SureFood® GMO ID 4plex Corn I |
The SureFood® GMO ID 4plex Corn I is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences: FAM channel: MON810 corn (OECD unique identifier MON-ØØ81Ø-6) VIC/HEX … Read more |
100 reactions | S2170 |
| SureFood® GMO ID 4plex Soya II |
SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below: Roundup Ready Soya (RR Soya) (OECD unique identifier … Read more |
100 reactions | S2162 |
| SureFood® GMO ID 4plex Canola II |
The multiplex test detects the following DNA-sequences of genetically modified canola: – FAM channel: MON88302 canola (OECD unique identifier MON-883Ø2-9) – VIC channel: Canola – ROX channel: DP73496 canola (OECD unique identifier … Read more |
100 reactions | S2167 |
| SureFood® GMO ID 4plex Canola I |
The multiplex test detects the following DNA sequences of genetically modified canola: FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8) ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7) Cy5 channel: T45 canola (OECD … Read more |
100 reactions | S2166 |
| SureFood® GMO ID 4plex Soya I |
The multiplex test SureFood® GMO ID 4plex Soya I detects the following DNA-sequences of soya: FAM-channel: MON87708 Soja (OECD Bezeichnung MON-877Ø8-9) FAM-channel: CV127 Soja (OECD Bezeichnung BPS-CV127-9) VIC-channel: DP305423 Soja (OECD … Read more |
100 reactions | S2161 |
| SureFood® GMO ID CV127 Soya (R&D Version) |
Detection of CV 127 Soya. Read more |
100 reactions | S2066 |
| SureFood® GMO ID DP305423 Soya (R&D Version) |
The test detects DP305423 Soya (OECD unique identifier DP-3Ø5423-1) DNA. The event specific detection is according to the official method of the European Commission. The real-time PCR assay can be used with established real-time PCR instruments … Read more |
100 reactions | S2065 |
| SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS |
This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. The multiplex test detects the following DNA-sequences: Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius … Read more |
100 reactions | S2127 |
| SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC |
The SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC is a real-time PCR for the direct, qualitative detection and differentiation of following specific DNA sequences. Read more |
100 reactions | S2158 |
| SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton |
The SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton is a real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola/cotton DNA sequences. Read more |
100 reactions | S2156 |
| SureFood® GMO QUANT GT73 Canola |
The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-ØØØ73-7) and the other one specific for Brassicacea family (reference … Read more |
2 x 50 reactions | S2061 |
| SureFood® GMO QUANT MON863 Corn |
The test detects the relative quantitative MON863 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON863 Corn (OECD unique identifier MON-ØØ863-5) and the other one specific for corn (the reference gene). The event … Read more |
2 x 50 reactions | S2051 |
| SureFood® GMO PLANT PLUS |
The test detects DNA of plants qualitatively. Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S2049 |
| SureFood® GMO QUANT MIR162 Corn |
The test detects the relative quantitative MIR162-Corn DNA amount. The kit contains two PCR systems, one event-specific for the MIR162-Corn (OECD unique identifier SYN-IR162-4) and the other one specific for Corn (reference system). Read more |
2 x 50 Reactions | S2135 |
| SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC |
This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S Cauliflower Mosaic Virus (CaMV) promoter DNA sequence, A. tumefaciens NOS terminator DNA … Read more |
100 reactions | S2126 |
| SureFood® GMO SCREEN CaMV |
The test detects CaMV DNA. The presence of CaMV DNA indicates an infestation with the cauliflower mosaic virus. Host of the CaMV are Brassicaceae plants. With the virus specific detection system SureFood® GMO CaMV it is possible to verify the … Read more |
100 reactions | S2027 |
| SureFood® GMO QUANT T25 Corn |
The test detects the relative quantitative T25-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the T25-Corn (OECD unique identifier ACS-ZMØØ3-2) and the other one specific for Corn (reference system). The … Read more |
100 reactions | S2017 |
| SureFood® GMO QUANT RR2Y Soya |
The test detects the relative quantitative RoundupReady2 Yield-Soya DNA amount (RR2Yield-Soya). Therefore the kit contains two PCR systems, one specific for the RR2Yield-Soya (OECD unique identifier MON-89788-1) and the other one specific for Soya … Read more |
2×50 reactions | S2029 |
| SureFood® GMO QUANT RoundUp Ready Soya |
The test detects the relative quantitative Roundup Ready Soya DNA amount (RR-Soya). Therefore the kit contains two PCR systems, one specific for the Roundup Ready Soya (OECD unique identifier MON-Ø4Ø32-6) and the other one specific for Soya … Read more |
2×50 reactions | S2014 |
| SureFood® GMO QUANT Bt176 Corn |
The test detects the relative quantitative Bt176-Corn DNA amount. Therefore the kit contains two PCR systems, one construct specific for the Bt176-Corn (OECD unique identifier SYN-EV176-9) and the other one specific for Corn (reference system). … Read more |
100 reactions | S2015 |
| SureFood® GMO QUANT Bt11 Corn |
The test detects the relative quantitative Bt11 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the event Bt11 Corn (OECD unique identifier SYN-BTØ11-1) and the other one specific for Corn (the reference gene). The … Read more |
2 x 50 reactions | S2016 |
| SureFood® GMO QUANT 35S Soya |
The test is developed for the relative estimation of 35S CaMV promoter DNA amount in only Soya containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Soya (the … Read more |
2×50 reactions | S2028 |
| SureFood® GMO QUANT 35S Corn |
The test is developed for the relative estimation of the 35S CaMV DNA amount in only Corn containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Corn (the … Read more |
2×50 reactions, whereof 50 reactions are used for the quantification of the reference gene. | S2020 |
| SureFood® GMO SCREEN P35S:BAR Rice |
The test detects the genetically construct P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OSØØ3-7) Rice and the LibertyLink 62 (LL62 – OECD unique identifier ACS-OSØØ2-5) Rice contains this genetically construct but … Read more |
100 reactions, whereas 50 reactions are used for detection of the reference gene. | S2022 |
| SureFood® GMO ID Bt63 Rice |
The test detects Bt63 Rice DNA. The detection of Bt63 Rice is a construct-specific real-time PCR assay. Additionally the SureFood® GMO ID Bt63 Rice kit contains a reference-PCR system for the detection of rice. The real time PCR system used in the … Read more |
100 reactions | S2024 |
