Pathogenic bacteria

Detect pathogens that can cause food poisoning

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Analytes

Pathogenic bacteria

Food contaminated with pathogenic bacteria may pose a serious health risk to the consumer. Thus the control of pathogens throughout the whole production chain is crucial to ensure consumer protection.

Pathogenic bacteria can trigger dangerous cases of food poisoning and must therefore not be present in food at all or at least a specific number per gram of food must not be exceeded. Particularly untreated fruits, vegetables, raw meat products, raw milk products as well as water are often sources for possible bacterial infections. Food safety is therefore a demanding challenge for all parties involved, especially in the area of quality assurance at primary production sites, food retailing and also for governmental authorities.

Together with Salmonellosis, Campylobacteriosis is one of the most significant diarrhoeal diseases worldwide. The strong increase of Campylobacter-enteritis is encouraged by the broad spread of the bacterium in different species of wild or farm animals as well as pets (birds and mammalians). As a commensal in the gut of poultry, Campylobacter reaches the food chain of humans via these animals. Additionally, other foodstuffs as milk, ground meat and drinking water can act as transmission pathways for the pathogens. Campylobacter is released by numerous hosts in high quantities to the environment and reaches the human body via contaminated foods. The infective dose to get to a Campylobacter enteritis is relatively low. It does not need more than 500 germs to get to an infection. From approximately 15 known Campylobacter species, mainly C. jejuni and C. coli are known to be causes for human gastroenteritis.

Clostridium species are gram-positive, spore-forming bacteria and are present almost everywhere in the environment. Some Clostridium species are of importance as pathogens, including Clostridium botulinum, Clostridium perfringens and Clostridium estertheticum. If food is not cooled sufficiently, it can result in a contamination, which can cause serious illnesses. The bacteria are heat-resistant and thus, can also be contained in thoroughly heated food products.

Legionella are a genus of gram-negative, rod-shaped bacteria that live in the water. They are considered as potential human pathogens. The Legionella species that is mostly responsible for illnesses in humans is Legionella pneumophila; it triggers the so-called legionnaire’s disease (legionellosis). The disease is usually transmitted through contaminated drinking water.

Listeria can be found in almost all areas of life. It is possible to assess the risk of contamination via quantitative detection to ensure that, if necessary, preventive measures can be taken. Listeria monocytogenes, in particular, is considered a major cause of food poisoning, possibly resulting in the development of sepsis, meningitis and encephalitis.

Salmonella is ranked among the most important initiators of cases of food poisoning. The WHO estimates more than 16 million global infections per year, and more than half a million cases are fatal. Salmonella are found in raw food such as eggs, meat and milk. The hazard potential is high, particularly in food that should only be slightly heated or not at all heated (e.g. raw milk cheese, raw sausage, chocolate, ice cream). In today’s mass animal farming industry, contaminations with Salmonella can hardly be avoided. Therefore, effective quality control with reliable test methods is important. For this purpose, we offer a series of different test systems, for the hygienic control of surfaces as well as for the analysis of food.

Staphylococcus aureus is a ball-shaped bacterium which can be observed as a grape-like clustered organism under the microscope. Cells are sized between 0.8 and 1.2 µm, Gram-positive and non-motile. S. aureus is a natural commensal of humans which lives on skin and upper airways and doesn’t cause diseases in general. In case of infections with pathogenic strains or in immunodeficient persons, S. aureus is able to cause skin infections and abscesses but also life-threatening diseases as pneumonia, meningitis, endocarditis, or toxic shock syndrome (TSS).

One reason for the facultative pathogenicity of S. aureus is its ability to form enterotoxins (SET), which can accumulate in contaminated food and cannot be inactivated totally by heat treatment. Contamination of foodstuffs with S. aureus is almost always caused by humans (hands, sneezing, coughing). S. aureus is therefore considered as indicator for poor personal hygiene.

Beside its relevance for food hygiene, S. aureus also plays a significant role in hospital hygiene. Especially strains of S. aureus which have acquired resistances against common antibiotics the so-called „methicillin resistant Staphylococcus aureus” (MRSA) might pose a non-assessable danger as they are difficult-to-treat.

The bacteria Escherichia coli (abbreviated E. coli) is a natural component of the intestinal flora in humans and animals and it is usually harmless. However, there are pathogenic strains that can cause serious infections. One of these pathogenic strains is known as enterohemorrhagic Escherichia E. coli (“EHEC”). These bacteria build the toxic substances verotoxin or shiga toxin and are also known as verotoxin-producing E. coli (“VTEC”) or shiga toxin-producing E. coli (“STEC”). Infections with STEC/VTEC are ranked as one of the most common food-related bacterial diseases that can even result in death. Sources of contamination mainly include raw meat and unpasteurized dairy products.

The group of Vibrio spp. includes pathogenic microorganisms which can cause serious infections after consumption of contaminated food. In diseases correlated to seafood, Vibrio spp. is one of the major causes of gastroenteritis, wound infection and sepsis. Additionally, in serious infections with the potential for epidemics such as Cholera, Vibrio spp. is causative.

The bacterium Vibrio parahaemolyticus naturally inhabits coastal waters and is present in higher concentrations during summer. The organism is halophilic or salt-requiring. Therefore, Vibrio parahaemolyticus is found in marine environments, seafood and the feces of patients with acute enteritis. Vibrio parahaemolyticus-associated gastroenteritis is the infection caused by this organism.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® SET Total (96 tests)

RIDASCREEN® SET Total is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures.

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Microtiter plate with 96 wells (12 strips with 8 breakable wells each),
96 determinations.
R4105
RIDASCREEN® SET A,B,C,D,E

RIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of Staphylococcus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures. Based on its sensitivity the RIDASCREEN® SET A,B,C,D,E test …

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Microtiter plate with 96 wells (12 strips with 8 wells each),
one strip is necessary for analysis of one sample,
12 tests in total.
R4101
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ProductDescriptionNo. of tests/amountArt. No.
Compact Dry LM

Usage of Compact Dry LM is a simple and safe test procedure for detection of Listeria monocytogenes in pre-enriched samples of foods. The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific nutrient pad. …

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HS9901: 100 nutrient plates,
HS9902: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9901 / HS9902
Compact Dry PA

Compact Dry PA is a simple and safe test procedure for the determination and quantification of Pseudomonas aeruginosa counts in foods, cosmetics, water samples or pharmaceutical materials. The ready-to-use plates consist of a special 50 mm diameter …

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HS9491: 100 nutrient plates,
HS9492: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9491 / HS9492
Compact Dry BC

Usage of Compact Dry BC is a simple and safe test procedure for determination and quantification of Bacillus cereus in foods or raw materials. The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific …

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HS9721: 100 nutrient plates,
HS9722: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9721 / HS9722
Compact Dry X-SA

Usage of Compact Dry X-SA is a simple and safe test procedure for determination and quantification of Staphylococcus aureus in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special …

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HS9621: 100 nutrient plates,
HS9622: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9621 / HS9622
Compact Dry VP

Usage of Compact Dry VP is a simple and safe test procedure for detection and quantification of Vibrio parahaemolyticus as well as Vibrio spp. in foods or raw materials. The ready-to-use plates consist of a special 50 mm diameter petri dish …

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HS8821: 100 nutrient plates,
HS8822: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS8821 / HS8822
Compact Dry SL

Usage of Compact Dry SL is a simple and safe test procedure for detection of Salmonella in pre-enriched samples of foods or raw materials. The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific nutrient …

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HS9401: 100 nutrient plates,
HS9402: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9401 / HS9402
Compact Dry LS

Usage of Compact Dry LS is a simple and safe test procedure for quantification of Listeria contaminations in foods or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special 50 mm diameter petri dish …

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HS8811: 100 nutrient plates,
HS8812: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS8811 / HS8812
Compact Dry EC

Usage of Compact Dry EC is a simple and safe test procedure for determination and quantification of coliform bacteria and E. coli in foods, cosmetics, water or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist …

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HS8781: 100 nutrient plates,
HS8782: 40 nutrient plates (HS8782),
four plates are packed in one aluminum bag.
HS8781 / HS8782
Compact Dry CF

Usage of Compact Dry CF is a simple and safe test procedure for determination and quantification of coliform bacteria in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special 50 mm …

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HS8791: 100 nutrient plates,
HS8792: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS8791 / HS8792
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ProductDescriptionNo. of tests/amountArt. No.
SureFast® Listeria 3plex ONE

The SureFast® Listeria 3plex ONE can be applied for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food. The method consists of the following three steps: 1) Cultural enrichment 2) …

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100 reactions F5217
SureFast® Campylobacter 4plex

The SureFast® Campylobacter 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Campylobacter jejuni, Campylobacter lari and Campylobacter coli.

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100 reactions F5170
SureFast® STEC 4plex ONE

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli …

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100 reactions F5265
SureFast® MRSA 4plex

The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of …

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100 reactions F7117
TANBead Maelstrom™ 8 Autostage

The TANBead Maelstrom™ 8 Autostage (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing …

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Up to 8 samples ZMAL8
SureFast® Yersinia 3plex

The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica.

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100 reactions F5132
SureFast® Escherichia coli Serotype II 4plex

The SureFast® Escherichia coli Serotype II 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific wzx gene DNA sequences of Escherichia coli O45, wbdl gene DNA sequences of Escherichia coli O111 and ihp1 gene …

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100 reactions F5168
SureFast® Escherichia coli Serotype I 4plex

The SureFast® Escherichia coli Serotype I 4plex is a real-time PCR kit for the direct, qualitative detection and differentiation of specfifc wzx gene DNA sequences of Escherichia coli O121, O26 and O103.

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100 reactions F5167
SureFast® Foodborne Pathogens 4plex

The SureFast® Foodborne Pathogens 4plex is a real-time PCR for for the direct, qualitative detection and differentiation of Escherichia coli virulence factors (stx1 [subtype a-d], stx2 [subtype a-g] and eae), Listeria monocytogenes and Salmonella …

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100 reactions F5175
SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex

The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium. Each reaction contains an internal …

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100 reactions F5166
SureFast®STEC Screening PLUS

The SureFast® STEC Screening PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the virulence factors stx1 (subtype a-d) and stx2 (subtype a-g) of Escherichia coli (STEC). Each reaction contains an internal …

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100 reactions F5105
SureFast® Parasitic Water Panel 4plex

The SureFast® Parasitic Water Panel 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Giardia intestinalis, Entamoeba histolytica and Cryptosporidium spp.. Each reaction contains an internal amplification control …

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100 reactions F5506
SureFast® Legionella 3plex

The SureFast® Legionella 3plex is a real-time PCR for the direct, qualitative detection of Legionella spp. and Legionella pneumophila. Each reaction contains an internal amplification control (IAC).

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100 reactions F5505
SureFast® Vibrio 4plex

The SureFast® Vibrio 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus DNA. Each reaction contains an internal amplification control (IAC).

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100 reactions F5161
SureFast® Pseudomonas aeruginosa PLUS

The SureFast® Pseudomonas aeruginosa PLUS is a real-time PCR for the direct, qualitative detection of a specific DNA sequence of Pseudomonas aeruginosa. Each reaction contains an internal amplification control (IAC).  

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100 reactions F5503
SureFast® Escherichia coli PLUS

The test detects Escherichia coli DNA. Each reaction contains an internal amplification control (IAC).  

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100 reactions F5157
SureFast® Cronobacter sakazakii PLUS

SureFast® Cronobacter sakazakii PLUS is a real-time PCR for the direct, qualitative detection of Cronobacter sakazakii DNA. Each reaction contains an internal amplification control (IAC).  

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100 reactions F5115
SureFast® Cronobacter PLUS

The test detects Cronobacter spp. DNA. Each reaction contains an internal amplification control (IAC).  

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100 reactions F5114
SureFast® Emetic Bacillus cereus PLUS

The SureFast® Emetic Bacillus cereus PLUS is a real-time PCR for the direct, qualitative detection of a specific cereulide synthetase DNA sequence of the emetic Bacillus cereus. Each reaction contains an internal amplification control (IAC).

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100 reactions F5127
SureFast® Clostridium perfringens PLUS

The SureFast® Clostridium perfringens PLUS is a real-time PCR for the direct, qualitative detection of a specific alpha-toxin DNA sequence of Clostridium perfringens. Each reaction contains an internal amplification control (IAC).  

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100 reactions F5123
SureFast® Clostridium botulinum Screening PLUS

The SureFast® Clostridium botulinum Screening PLUS is a real-time PCR for the direct, qualitative detection of specific Botulinum neurotoxins (BoNT) A, B, E and F DNA sequences of Clostridium botulinum, Clostridium baratii and Clostridium butyricum. …

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100 reactions F5110
SureFast® Clostridium estertheticum PLUS

The test detects Clostridium estertheticum DNA. Each reaction contains an internal amplification control (IAC).

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100 reactions F5160
SureFast® Salmonella ONE

Salmonella spp. detection with real-time PCR Two in one – Salmonella test kit with included DNA preparation The kit is intended to be used for the fast and simple isolation and detection of Salmonella DNA from enrichment cultures. Each reaction …

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100 reactions F5211
SureFast® Salmonella PLUS

The SureFast® Salmonella PLUS is a real-time PCR for the direct, qualitative detection of a specific DNA sequence of Salmonella spp.. The SureFast® Salmonella PLUS real-time PCR kit has been validated and certified in combination with the …

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100 reactions F5111
SureFast® EHEC/EPEC 4plex

SureFast® EHEC/EPEC 4plex is a real-time PCR for the direct, qualitative detection and differentiation of DNA sequences of the virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the E. coli and Shigella spp. virulence factor ipaH. The …

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100 reactions F5128
SureFast® Legionella Screen PLUS

The test detects Legionella spp. DNA. Each reaction contains an internal amplification control (IAC).  

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100 reactions F5502
SureFast® Bacillus cereus group PLUS

The SureFast® Bacillus cereus group PLUS is a real-time PCR for the direct, qualitative detection of specific DNA sequences of Bacillus cereus group (Bacillus anthracis, Bacillus cereus, Bacillus cytotoxis, Bacillus mycoides, Bacillus …

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100 reactions F5126
SureFast® Staphylococcus aureus PLUS

The SureFast® Staphylococcus aureus PLUS is a real-time PCR for the direct, qualitative detection of a specific Staphylococcus aureus DNA sequence. Each reaction contains an internal amplification control (IAC).

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100 reactions F5116
SureFast® Listeria monocytogenes PLUS

SureFast® Listeria monocytogenes PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (IAC).

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100 reactions F5113
SureFast® Listeria Screening PLUS

The SureFast® Listeria Screening PLUS is a real-time PCR for the direct, qualitative detection of a specific DNA sequence of Listeria spp.. Each reaction contains an internal amplification control (IAC).

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100 reactions F5117
SureFast® Legionella pneumophila PLUS

The test detects Legionella pneumophila DNA. Each reaction contains an internal amplification control (IAC).

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100 reactions F5501
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