ELISA
Efficient immunoassays for food analysis
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ELISA
The enzyme-linked immunosorbent assay (ELISA) is an antibody-based test method. This widespread technology is sensitive, fast and reliable.
With their ELISA test kits, R-Biopharm relies on a well-established analytical method that meets the requirements of modern laboratories. RIDASCREEN® tests show a high sensitivity and are characterized by robustness. For the time-sensitive screening of various commodities, RIDASCREEN®FAST tests are offered. These tests enable to analyze food and feed samples within a maximum of 30 min including sample preparation.
ELISA principle
Antibodies are proteins produced in plasma cells of vertebrates as part of the adaptive immune system against structures (antigens) which are recognized as foreign to the body. Antibodies bind to their antigens by a distinct pattern of ionic and hydrophobic interactions, hydrogen bridge linkages and Van-der Waals forces. The interaction between antibody and its antigen is selective and highly specific, similar to a lock and key.
The enzyme-linked immunosorbent assay is based on this selective and specific antibody-antigen recognition. Many formats of qualitative or quantitative ELISA tests have been established. Performing an ELISA test involves at least one specific antibody for a particular antigen. A main principle is that one of these immunological components is immobilized to a solid phase, the cavities of a microtiter plate. The analyte from the sample interacts with the antibody-antigen system. This interaction can be visualized by enzymes, linked to secondary antibodies or antigens, and indicate if an antibody-antigen binding has occurred. An added substrate is converted by the coupled enzyme resulting in a change of colour, which can be measured with a spectrophotometer.
Sandwich ELISA and competitive ELISA
While a sandwich ELISA detects only intact proteins and large fragments with at least two binding sites, a competitive ELISA is able to detect large and small protein fragments with only one binding site. For this reason, the competitive ELISA can also be used for highly processed food products.
Good ELISA practice
The manual provides a comprehensive overview for both – beginners and advanced analysts, in order to improve the quality of performed ELISA analysis. It represents a guideline which will help you to establish reasonable framework conditions and conditions of use, which shall be complied with to achieve the best possible performance when using R-Biopharm ELISA test kits.
Product portfolio
Product | Description | No. of tests/amount | Art. No. |
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RIDASCREEN® Risk Material 10/5 |
RIDASCREEN® Risk Material 10/5 is a sandwich enzyme immunoassay for the quantitative analysis of risk material (CNS) in / on raw meat, meat products and on contaminated surfaces. For the determination of risk material (CNS) in processed (heated) … Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R6703 |
RIDASCREEN® Risk Material |
RIDASCREEN® Risk Material is a sandwich enzyme immunoassay for the quantitative analysis of risk material (CNS) in processed (heated) meat and sausages. For the determination of risk material (CNS) in raw meat, meat products and on contaminated … Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R6701 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN®FAST Hazelnut |
RIDASCREEN®FAST Hazelnut is a sandwich enzyme immunoassay for the quantitative analysis of hazelnut (e.g. traces of hazelnut) in food such as cereals, bakery products, ice cream and chocolate. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6802 |
RIDASCREEN®FAST Sesame |
RIDASCREEN®FAST Sesame is a sandwich enzyme immunoassay for the quantitative analysis of sesame or parts of sesame (as ingredient components or contamination) in instant soup, chocolate dessert, bakery products and crackers. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7202 |
RIDASCREEN®FAST Soya |
RIDASCREEN®FAST Soya is a sandwich enzyme immunoassay for the quantitative analysis of native and processed soya proteins in food. Such products are beverages and food as sausages, dressings, bakery products, ice, chocolate, soup, sauce, margarine, … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7102 |
RIDASCREEN®FAST Milk |
RIDASCREEN®FAST Milk is a sandwich enzyme immunoassay to quantify milk proteins (casein and ß-lactoglobulin) in food containing whey, milk or milk powder such as sausages, ice cream, chocolate, bakery goods, cake and bread mix, soups, sauces, … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R4652 |
RIDASCREEN® Histamin |
RIDASCREEN® Histamin is a competitive enzyme immunoassay for the quantitative analysis of histamine in white and red wine, sparkling wine, milk, cheese, fresh fish, canned fish and fish meal. Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R1601 |
RIDASCREEN®FAST β-Lactoglobulin |
RIDASCREEN®FAST β-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate and sausage. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R4912 |
RIDASCREEN® β-Lactoglobulin |
RIDASCREEN® β-Lactoglobulin is a competitive enzyme immunoassay for the quantitative analysis of β-lactoglobulin in hydrolyzed milk products including hypoallergenic baby food. The assay is calibrated to hydrolyzed β-lactoglobulin check … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R4901 |
RIDASCREEN®FAST Mustard |
RIDASCREEN®FAST Mustard is a sandwich enzyme immunoassay for the quantitative analysis of mustard in ketchup, sausage and cream cheese. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6152 |
RIDASCREEN®FAST Lysozym |
RIDASCREEN®FAST Lysozym is a sandwich enzyme immunoassay for the quantitative analysis of lysozyme (hen’s egg protein) in food like wine, cheese and sausage. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6452 |
RIDASCREEN®FAST Lupine |
RIDASCREEN®FAST Lupine is a sandwich enzyme immunoassay for the quantitative analysis of lupine proteins in food (e.g. juices, wine, beer, sausages, noodles, chocolate, bakery products or ice cream). Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6102 |
RIDASCREEN® Gliadin competitive |
RIDASCREEN® Gliadin competitive is used for the analysis of fermented and hydrolyzed food (e.g. beer, starch syrup, starch, malt extract, sourdough, soy sauce) which are declared as “gluten-free”. The R5 monoclonal antibody recognizes … Read more |
Microtiter plate with 96 wells (12 strips with 8 removable wells each) | R7021 |
RIDASCREEN®FAST Gliadin |
RIDASCREEN® FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7002 |
RIDASCREEN®FAST Almond |
RIDASCREEN®FAST Almond is a sandwich enzyme immunoassay for the quantitative analysis of almond or parts of almond in food. As representative samples for the food groups pastries and sweets the following matrices have been tested during the assay´s … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6901 |
RIDASCREEN®FAST Egg Protein |
RIDASCREEN®FAST Egg Protein is a sandwich enzyme immunoassay for the quantitative analysis of whole egg (-powder) in food like salad dressings, sausages, wines, baking-mixtures for cakes or bread and ice cream. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R6402 |
RIDASCREEN®FAST Crustacean |
RIDASCREEN®FAST Crustacean is a sandwich enzyme immunoassay for the quantitative analysis of crustaceans which can be present as ingredient or contamination in raw or cooked food. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R7312 |
RIDASCREEN®FAST Casein |
RIDASCREEN®FAST Casein is a sandwich enzyme immunoassay for the quantitative analysis of casein in food like bakery goods, cake and bread mix, non-hydrolyzed milk-based infant formula, ice cream, beverages, chocolate, wine and sausages. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R4612 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® SET Total (96 tests) |
RIDASCREEN® SET Total is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures. Read more |
Microtiter plate with 96 wells (12 strips with 8 breakable wells each), 96 determinations. |
R4105 |
RIDASCREEN® SET A,B,C,D,E |
RIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of Staphylococcus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures. Based on its sensitivity the RIDASCREEN® SET A,B,C,D,E test … Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each), one strip is necessary for analysis of one sample, 12 tests in total. |
R4101 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN®FAST Zearalenon SC |
RIDASCREEN®FAST Zearalenon SC is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R5505 |
RIDASCREEN®FAST Zearalenon |
RIDASCREEN®FAST Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals and feed. The test was validated with different matrices of cereals and composed feed. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R5502 |
RIDASCREEN®FAST T-2 Toxin |
RIDASCREEN®FAST T-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of T-2 toxin in cereals and feed. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R5302 |
RIDASCREEN®FAST Ochratoxin A |
RIDASCREEN®FAST Ochratoxin A is a competitive enzyme immunoassay for the quantitative analysis of ochratoxin A in corn, wheat, barley, oats (grains) and feed. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R5402 |
RIDASCREEN®FAST DON SC |
RIDASCREEN®FAST DON SC is a competitive enzyme immunoassay for the quantitative determination of DON in cereals, malt and feed with a sinlge control standard curve. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each). | R5905 |
RIDASCREEN®FAST DON |
RIDASCREEN®FAST DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt and feed. Read more |
R5901: microtiter plate with 96 wells (12 strips with 8 removable wells each) R5902: microtiter plate with 48 wells (6 strips with 8 removable wells each) |
R5901 / R5902 |
RIDASCREEN®FAST Citrinin |
RIDASCREEN®FAST Citrinin assay is a competitive enzyme immunoassay for the quantitative determination of citrinin in cereals and feed. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R6302 |
RIDASCREEN®FAST Aflatoxin SC |
RIDASCREEN®FAST Aflatoxin SC is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed with a single control standard curve. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R9002 |
RIDASCREEN®FAST Aflatoxin |
RIDASCREEN®FAST Aflatoxin is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed. Read more |
Microtiter plate with 48 wells (6 strips with 8 wells each) | R5202 |
RIDASCREEN® Aflatoxin Total |
RIDASCREEN® Aflatoxin Total is a competitive enzyme immunoassay for the quantitative determination of aflatoxins in cereals and feed. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R4701 |
RIDASCREEN® Aflatoxin B1 30/15 |
RIDASCREEN® Aflatoxin B1 30/15 is a competitive enzyme immunoassay for the quantitative determination of aflatoxin B1 in cereals and feed. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1211 |
RIDASCREEN® Aflatoxin M1 |
RIDASCREEN® Aflatoxin M1 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk and milk powder. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1121 |
RIDASCREEN®FAST Fumonisin ECO |
RIDASCREEN®FAST Fumonisin ECO is a competitive enzyme immunoassay for the quantitative determination of fumonisin in corn and feed. Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R5603 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN® Clenbuterol |
RIDASCREEN® Clenbuterol is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in milk, meat, liver, kidney, urine, plasma/serum, hair, eyeball and feed. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1711 |
RIDASCREEN® β-Agonists |
RIDASCREEN® β-Agonists is a competitive enzyme immunoassay for the quantitative analysis of β-Agonists in urine, serum, meat, liver, milk and feed. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R1704 |
RIDASCREEN® Ractopamin |
RIDASCREEN® Ractopamin is a competitive enzyme immunoassay for the quantitative analysis of ractopamine in urine, meat and liver and for the qualitative analysis of ractopamin glucuronide in urine. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R9901 |
RIDASCREEN® Sulfonamide |
RIDASCREEN® Sulfonamide is a competitive enzyme immunoassay for the quantitative analysis of sulfonamides in egg, meat (chicken and pork), fish, shrimp, honey and milk. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each). | R3004 |
RIDASCREEN® Nitrofuran (AHD) |
RIDASCREEN® Nitrofuran (AHD) is a competitive enzyme immunoassay for the quantitative analysis of the nitrofurantoin metabolite AHD in shrimp and fish. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3713 |
RIDASCREEN® Nitrofuran (AMOZ) |
RIDASCREEN® Nitrofuran (AMOZ) is a competitive enzyme immunoassay for the quantitative determination of AMOZ in shrimp, fish, meat and poultry. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3722 |
RIDASCREEN® Nitrofuran (AOZ) |
RIDASCREEN® Nitrofuran (AOZ) is a competitive enzyme immunoassay for the quantitative analysis of AOZ in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3703 |
RIDASCREEN® Chinolone/Quinolones |
RIDASCREEN® Chinolone/Quinolones is a competitive enzyme immunoassay for the quantitative analysis of chinolones in egg, meat (beef, pork, sheep, chicken, turkey), fish and shrimp. Read more |
Microtiter plate with 96 wells (12 strips with 8 wells each) | R3113 |
Product | Description | No. of tests/amount | Art. No. |
---|---|---|---|
RIDASCREEN®FAST Folic acid |
RIDASCREEN®FAST Folic acid is a competitive enzyme immunoassay for the quantitative determination of added folic acid in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets and … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R3203 |
RIDASCREEN®FAST Vitamin B12 |
RIDASCREEN®FAST Vitamin B12 is a competitive enzyme immunoassay for the quantitative determination of vitamin B12 in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets, and … Read more |
Microtiter plate with 48 wells (6 strips with 8 removable wells each) | R2103 |