ELISA

Efficient immunoassays for food analysis

Technologies

ELISA

The enzyme-linked immunosorbent assay (ELISA) is an antibody-based test method. This widespread technology is sensitive, fast and reliable.

With their ELISA test kits, R-Biopharm relies on a well-established analytical method that meets the requirements of modern laboratories. RIDASCREEN® tests show a high sensitivity and are characterized by robustness. For the time-sensitive screening of various commodities, RIDASCREEN®FAST tests are offered. These tests enable to analyze food and feed samples within a maximum of 30 min including sample preparation.

ELISA principle

Antibodies are proteins produced in plasma cells of vertebrates as part of the adaptive immune system against structures (antigens) which are recognized as foreign to the body. Antibodies bind to their antigens by a distinct pattern of ionic and hydrophobic interactions, hydrogen bridge linkages and Van-der Waals forces. The interaction between antibody and its antigen is selective and highly specific, similar to a lock and key.

The enzyme-linked immunosorbent assay is based on this selective and specific antibody-antigen recognition. Many formats of qualitative or quantitative ELISA tests have been established. Performing an ELISA test involves at least one specific antibody for a particular antigen. A main principle is that one of these immunological components is immobilized to a solid phase, the cavities of a microtiter plate. The analyte from the sample interacts with the antibody-antigen system. This interaction can be visualized by enzymes, linked to secondary antibodies or antigens, and indicate if an antibody-antigen binding has occurred. An added substrate is converted by the coupled enzyme resulting in a change of colour, which can be measured with a spectrophotometer.

Sandwich ELISA and competitive ELISA

While a sandwich ELISA detects only intact proteins and large fragments with at least two binding sites, a competitive ELISA is able to detect large and small protein fragments with only one binding site. For this reason, the competitive ELISA can also be used for highly processed food products.

Good ELISA practice

The manual provides a comprehensive overview for both – beginners and advanced analysts, in order to improve the quality of performed ELISA analysis. It represents a guideline which will help you to establish reasonable framework conditions and conditions of use, which shall be complied with to achieve the best possible performance when using R-Biopharm ELISA test kits.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Risk Material 10/5

RIDASCREEN® Risk Material 10/5 is a sandwich enzyme immunoassay for the quantitative analysis of risk material (CNS) in / on raw meat, meat products and on contaminated surfaces. For the determination of risk material (CNS) in processed (heated) …

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Microtiter plate with 96 wells (12 strips with 8 wells each) R6703
RIDASCREEN® Risk Material

RIDASCREEN® Risk Material is a sandwich enzyme immunoassay for the quantitative analysis of risk material (CNS) in processed (heated) meat and sausages. For the determination of risk material (CNS) in raw meat, meat products and on contaminated …

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Microtiter plate with 96 wells (12 strips with 8 wells each). R6701
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN®FAST Hazelnut

RIDASCREEN®FAST Hazelnut is a sandwich enzyme immunoassay for the quantitative analysis of hazelnut (e.g. traces of hazelnut) in food such as cereals, bakery products, ice cream and chocolate.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6802
RIDASCREEN®FAST Sesame

RIDASCREEN®FAST Sesame is a sandwich enzyme immunoassay for the quantitative analysis of sesame or parts of sesame (as ingredient components or contamination) in instant soup, chocolate dessert, bakery products and crackers.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R7202
RIDASCREEN®FAST Soya

RIDASCREEN®FAST Soya is a sandwich enzyme immunoassay for the quantitative analysis of native and processed soya proteins in food. Such products are beverages and food as sausages, dressings, bakery products, ice, chocolate, soup, sauce, margarine, …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R7102
RIDASCREEN®FAST Milk

RIDASCREEN®FAST Milk is a sandwich enzyme immunoassay to quantify milk proteins (casein and ß-lactoglobulin) in food containing whey, milk or milk powder such as sausages, ice cream, chocolate, bakery goods, cake and bread mix, soups, sauces, …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R4652
RIDASCREEN® Histamin

RIDASCREEN® Histamin is a competitive enzyme immunoassay for the quantitative analysis of histamine in white and red wine, sparkling wine, milk, cheese, fresh fish, canned fish and fish meal.

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R1601
RIDASCREEN®FAST β-Lactoglobulin

RIDASCREEN®FAST β-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate and sausage.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R4912
RIDASCREEN® β-Lactoglobulin

RIDASCREEN® β-Lactoglobulin is a competitive enzyme immunoassay for the quantitative analysis of β-lactoglobulin in hydrolyzed milk products including hypoallergenic baby food. The assay is calibrated to  hydrolyzed β-lactoglobulin check …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R4901
RIDASCREEN®FAST Mustard

RIDASCREEN®FAST Mustard is a sandwich enzyme immunoassay for the quantitative analysis of mustard in ketchup, sausage and cream cheese.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6152
RIDASCREEN®FAST Lysozym

RIDASCREEN®FAST Lysozym is a sandwich enzyme immunoassay for the quantitative analysis of lysozyme (hen’s egg protein) in food like wine, cheese and sausage.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6452
RIDASCREEN®FAST Lupine

RIDASCREEN®FAST Lupine is a sandwich enzyme immunoassay for the quantitative analysis of lupine proteins in food (e.g. juices, wine, beer, sausages, noodles, chocolate, bakery products or ice cream).

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6102
RIDASCREEN® Gliadin competitive

RIDASCREEN® Gliadin competitive is used for the analysis of fermented and hydrolyzed food (e.g. beer, starch syrup, starch, malt extract, sourdough, soy sauce) which are declared as “gluten-free”. The R5 monoclonal antibody recognizes …

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Microtiter plate with 96 wells (12 strips with 8 removable wells each) R7021
RIDASCREEN®FAST Gliadin

RIDASCREEN® FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R7002
RIDASCREEN®FAST Almond

RIDASCREEN®FAST Almond is a sandwich enzyme immunoassay for the quantitative analysis of almond or parts of almond in food. As representative samples for the food groups pastries and sweets the following matrices have been tested during the assay´s …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6901
RIDASCREEN®FAST Egg Protein

RIDASCREEN®FAST Egg Protein is a sandwich enzyme immunoassay for the quantitative analysis of whole egg (-powder) in food like salad dressings, sausages, wines, baking-mixtures for cakes or bread and ice cream.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R6402
RIDASCREEN®FAST Crustacean

RIDASCREEN®FAST Crustacean is a sandwich enzyme immunoassay for the quantitative analysis of crustaceans which can be present as ingredient or contamination in raw or cooked food.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R7312
RIDASCREEN®FAST Casein

RIDASCREEN®FAST Casein is a sandwich enzyme immunoassay for the quantitative analysis of casein in food like bakery goods, cake and bread mix, non-hydrolyzed milk-based infant formula, ice cream, beverages, chocolate, wine and sausages.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R4612
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® SET Total (96 tests)

RIDASCREEN® SET Total is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures.

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Microtiter plate with 96 wells (12 strips with 8 breakable wells each),
96 determinations.
R4105
RIDASCREEN® SET A,B,C,D,E

RIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of Staphylococcus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures. Based on its sensitivity the RIDASCREEN® SET A,B,C,D,E test …

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Microtiter plate with 96 wells (12 strips with 8 wells each),
one strip is necessary for analysis of one sample,
12 tests in total.
R4101
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN®FAST Zearalenon SC

RIDASCREEN®FAST Zearalenon SC is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5505
RIDASCREEN®FAST Zearalenon

RIDASCREEN®FAST Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals and feed. The test was validated with different matrices of cereals and composed feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5502
RIDASCREEN®FAST T-2 Toxin

RIDASCREEN®FAST T-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of T-2 toxin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5302
RIDASCREEN®FAST Ochratoxin A

RIDASCREEN®FAST Ochratoxin A is a competitive enzyme immunoassay for the quantitative analysis of ochratoxin A in corn, wheat, barley, oats (grains) and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5402
RIDASCREEN®FAST DON SC

RIDASCREEN®FAST DON SC is a competitive enzyme immunoassay for the quantitative determination of DON in cereals, malt and feed with a sinlge control standard curve.

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Microtiter plate with 48 wells (6 strips with 8 wells each). R5905
RIDASCREEN®FAST DON

RIDASCREEN®FAST DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt and feed.

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R5901: microtiter plate with 96 wells (12 strips with 8 removable wells each)
R5902: microtiter plate with 48 wells (6 strips with 8 removable wells each)
R5901 / R5902
RIDASCREEN®FAST Citrinin

RIDASCREEN®FAST Citrinin assay is a competitive enzyme immunoassay for the quantitative determination of citrinin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R6302
RIDASCREEN®FAST Aflatoxin SC

RIDASCREEN®FAST Aflatoxin SC is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed with a single control standard curve.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R9002
RIDASCREEN®FAST Aflatoxin

RIDASCREEN®FAST Aflatoxin is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5202
RIDASCREEN® Aflatoxin Total

RIDASCREEN® Aflatoxin Total is a competitive enzyme immunoassay for the quantitative determination of aflatoxins in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R4701
RIDASCREEN® Aflatoxin B1 30/15

RIDASCREEN® Aflatoxin B1 30/15 is a competitive enzyme immunoassay for the quantitative determination of aflatoxin B1 in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1211
RIDASCREEN® Aflatoxin M1

RIDASCREEN® Aflatoxin M1 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk and milk powder.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1121
RIDASCREEN®FAST Fumonisin ECO

RIDASCREEN®FAST Fumonisin ECO is a competitive enzyme immunoassay for the quantitative determination of fumonisin in corn and feed.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R5603
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Clenbuterol

RIDASCREEN® Clenbuterol is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in milk, meat, liver, kidney, urine, plasma/serum, hair, eyeball and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1711
RIDASCREEN® β-Agonists

RIDASCREEN® β-Agonists is a competitive enzyme immuno­assay for the quantitative analysis of β-Agonists in urine, serum, meat, liver, milk and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1704
RIDASCREEN® Ractopamin

RIDASCREEN® Ractopamin is a competitive enzyme immunoassay for the quantitative analysis of ractopamine in urine, meat and liver and for the qualitative analysis of ractopamin glucuronide in urine.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R9901
RIDASCREEN® Sulfonamide

RIDASCREEN® Sulfonamide is a competitive enzyme immunoassay for the quantitative analysis of sulfonamides in egg, meat (chicken and pork), fish, shrimp, honey and milk.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R3004
RIDASCREEN® Nitrofuran (AHD)

RIDASCREEN® Nitrofuran (AHD) is a competitive enzyme immunoassay for the quantitative analysis of the nitrofurantoin metabolite AHD in shrimp and fish.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3713
RIDASCREEN® Nitrofuran (AMOZ)

RIDASCREEN® Nitrofuran (AMOZ) is a competitive enzyme immunoassay for the quantitative determination of AMOZ in shrimp, fish, meat and poultry.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3722
RIDASCREEN® Nitrofuran (AOZ)

RIDASCREEN® Nitrofuran (AOZ) is a competitive enzyme immunoassay for the quantitative analysis of AOZ in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3703
RIDASCREEN® Chinolone/Quinolones

RIDASCREEN® Chinolone/Quinolones is a competitive enzyme immunoassay for the quantitative analysis of chinolones in egg, meat (beef, pork, sheep, chicken, turkey), fish and shrimp.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3113
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN®FAST Folic acid

RIDASCREEN®FAST Folic acid is a competitive enzyme immunoassay for the quantitative determination of added folic acid in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets and …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R3203
RIDASCREEN®FAST Vitamin B12

RIDASCREEN®FAST Vitamin B12 is a competitive enzyme immunoassay for the quantitative determination of vitamin B12 in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets, and …

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R2103
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