Real-time PCR
Molecular diagnostics in food analysis
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Real-time PCR
In the field of food analysis, qPCR tests are indispensable, offering a multitude of applications. qPCR tests stand out for their specificity, sensitivity, and robustness. They deliver rapid and reliable results, and best of all, these tests can be semi-automated, streamlining your workflow and enhancing efficiency.
Our qPCR solutions feature standardized protocols and provide a seamless, all-in-one experience. From sample preparation—whether manual or automated—to final analysis, we cover every step. With our DNA preparation, you can perform multiple qPCR runs or analyses from a single sample, maximizing efficiency and resource utilization. We offer a wide array of qPCR kits as well as the RIDA®CYCLER customized to suit your requirements.
Broad Multiplex PCR Kit Range: Our extensive selection of multiplex qPCR kits allows for the simultaneous detection of multiple targets in a single reaction, enhancing efficiency and reducing costs.
The modular system of DNA preparation and DNA detection can be used independently. Moreover, it is an open system: Compatible with various PCR platforms (FAM/HEX/ROX/Cy5), offering flexibility and convenience for your laboratory setup.
Contact us to learn more about our PCR solutions and how they can benefit your lab or food production facility. Our team offers comprehensive support and training options to ensure you get the most out of our products.
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Real-time PCR explained
The first step of all analysis is representative sampling and homogenization. After lysis of the organic cells of food tissue, the DNA will be prepared using different dedicated DNA or RNA preparation kits either for bacteria or viruses.
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Manual vs. automated nucleic acid preparation
What is more convenient – manual or automated? This video clearly compares the individual steps of manual and automatic (with TANBead Maelstrom™ 4800) nucleic acid preparation.
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The multiplex real-time PCR instrument
The compact RIDA®CYCLER is a real-time PCR cycler based on magnetic induction with 4 channels. The user-friendly software, pre-installed with all SureFast® and SureFood® thermal profiles, allows a fast analysis start of one or more tests simultaneously. This compact and easy to use real-time cycler allows fast and flexible analysis of your food & feed samples.
Application areas for PCR in food testing
Real-time PCR Product Overview
- 25 Single target kits: Detect specific allergens such as celery, mustard, fish, molluscs, crustaceans, soya, sesame, hazelnut, peanut or almond with precision.
- 8 Multiplex kits: Simultaneously detect multiple allergens, including combinations like celery/soya/mustard, fish/molluscs/crustaceans or various nuts combinations ensuring comprehensive testing in one go.
- Lab reference material for quantification.
- SureFast® product line: Detect a wide range of microbiological contaminants, including pathogenic bacteria (e.g. Salmonella, Listeria, E. coli, Campylobacter) and viruses (e.g. Norovirus, Hepatitis).
- SureFast® ONE: This all-in-one kit includes both DNA preparation and qPCR components, featuring fast DNA prep and easy-to-use protocols, streamlining your testing process.
- Water analysis: Detect Legionella, Pseudomonas aeruginosa, and other waterborne pathogens, including a parasitic water panel and screening kits for comprehensive water safety analysis.
Our SureFood® GMO product line offers both qualitative and quantitative detection of GMOs.
- Designed for flexibility, our kits support both manual and semi-automated workflows.
- Comprehensive screening: Begin with unspecific multiplex screening.
- Precise identification: Follow up with multiplex identification of specific GMOs.
- Relative quantification: Conclude with the relative quantification of EU authorized GMOs, ensuring compliance.
Species specification is of great importance in the food industry. Additionally, ensuring compliance with religious dietary rules (halal, kosher) is crucial. Our SureFood® Animal ID product line offers highly sensitive testing for various species, including pork, beef, horse, donkey, chicken, turkey, and more.
- Multiplex options: Our kits provide multiplex testing capabilities, allowing for the simultaneous detection of multiple species in a single assay, saving time and resources.
- SureFood® ANIMAL ID Pork SENS PLUS: Our specialized pork detection kit is designed for exceptional sensitivity, ensuring accurate identification even at low levels of contamination.
Our GEN-IAL® product line offers a broad range of multiplex kits designed to detect spoilage organisms in beverages such as beer, wine, juice, and alcohol-free products.
- Broad range of multiplex kits: Efficiently detect multiple bacteria and yeast species simultaneously, ensuring comprehensive testing for beverage spoilage organisms.
- One DNA preparation protocol for bacteria and yeast: Simplify your workflow with a single preparation process for both bacteria and yeast detection.
- Pre-coated tubes for easy handling: Our kits come with pre-coated tubes, ensuring ease of use and reducing the risk of contamination.
- Open system: Compatible with various PCR platforms, offering flexibility and convenience for your laboratory setup.
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Read more |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Read more |
96 preparations | F1060 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7007 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® ALLERGEN 4plex SEAFOOD |
The SureFood® ALLERGEN 4plex SEAFOOD is a multiplex real-time PCR for the qualitative detection and differentiation of specific fish, crustaceans and molluscs DNA. Read more |
100 reactions | S3405 |
| SureFood® ALLERGEN Coconut |
The SureFood® ALLERGEN Coconut is a real-time PCR for the direct, qualitative detection of specific coconut (Cocos nucifera) DNA sequences in food. Read more |
100 reactions | S3621 |
| SureFood® ALLERGEN 4plex Almond/Pistachio/Cashew+IAC |
The SureFood® ALLERGEN 4plex Almond/Pistachio/Cashew+IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific almond (Prunus dulcis), pistachio (Pistacia vera) and cashew (Anacardium occidentale) DNA … Read more |
100 reactions | S3406 |
| SureFood® ALLERGEN 4plex EU NUTS |
The SureFood® ALLERGEN 4plex EU NUTS is a multiplex real-time PCR for the direct, qualitative detection and differentiation of almond (Prunus dulcis), cashew (Anacardium occidentale), pistachio (Pistacia vera), peanut (Arachis hypogaea), hazelnut … Read more |
100 reactions | S3404 |
| SureFood® 4plex LEGUMES |
The SureFood® 4plex LEGUMES is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of legumes (Fabaceae), bean (Phaseolus spp. and Vigna spp.) and pea (Pisum sativum). Read more |
100 reactions | S7008 |
| SureFood® ALLERGEN Shea Nut |
The SureFood® ALLERGEN Shea Nut is a real-time PCR for the direct, qualitative detection of specific shea nut (Vitellaria paradoxa) DNA sequences in raw material. Read more |
100 reactions | S3622 |
| SureFood® ALLERGEN Beechnut |
The SureFood® ALLERGEN Beechnut is a real-time PCR for the direct qualitative detection of specific beechnut (Fagus sylvatica) DNA sequences in food. Read more |
100 reactions | S3628 |
| SureFood® ALLERGEN Insects |
The SureFood® ALLERGEN Insects is a real-time PCR for the direct, qualitative detection of specific insects (Insecta) DNA sequences. Read more |
100 reactions | S3626 |
| SureFood® ALLERGEN Pine Nut |
The SureFood® ALLERGEN Pine Nut is a real-time PCR for the direct, qualitative detection of specific pine nut (Pinus spp.) DNA sequences in food. Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S3624 |
| SureFood® ALLERGEN Buckwheat |
The SureFood® ALLERGEN Buckwheat is a real-time PCR for the direct, qualitative and / or quantitative detection of specific buckwheat (Fagopyrum esculentum) DNA sequences. For the quantitative determination the use of the laboratory reference … Read more |
100 reactions | S3620 |
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Read more |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Read more |
96 preparations | F1060 |
| SureFood® ALLERGEN 4plex Cereals |
The SureFood® ALLERGEN 4plex Cereals is a multiplex real-time PCR test for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences in food. Each reaction … Read more |
100 reactions | S7006 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7007 |
| SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC |
The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya … Read more |
100 reactions | S3403 |
| SureFood® ALLERGEN Oat |
The real-time PCR test detects DNA of oat (Avena sativa) qualitatively. Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7004 |
| SureFood® ALLERGEN Mustard |
The SureFood® ALLERGEN Mustard is a real-time PCR for the direct, qualitative and / or quantitative detection of specific white mustard (Sinapis alba), indian mustard (Brassica juncea) und black mustard (Brassica nigra) DNA sequences according to … Read more |
100 reactions | S3609 |
| SureFood® ALLERGEN Lupin |
The real-time PCR test detects DNA of lupin (Lupinus spp.) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of the … Read more |
100 reactions | S3611 |
| SureFood® ALLERGEN Celery |
The real-time PCR test detects DNA of celery (Apium graveolens) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3605 |
| SureFood® ALLERGEN Molluscs |
The SureFood® ALLERGEN Molluscs is a real-time PCR for the direct, qualitative and / or quantitative detection of specific molluscs (mollusca) DNA sequences according to directive (EC) 1169/2011 in food. The molluscs family contains 50,000 – … Read more |
100 reactions | S3613 |
| SureFood® ALLERGEN Fish |
The SureFood® ALLERGEN Fish is a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large … Read more |
100 reactions | S3610 |
| SureFood® ALLERGEN Crustaceans |
The SureFood® ALLERGEN Crustaceans is a real-time PCR for the direct, qualitative and / or quantitative detection of specific crustaceans (Crustacea) DNA sequences according to directive (EC) 1169/2011. The crustacean family contains about 44,000 … Read more |
100 reactions | S3612 |
| SureFood® ALLERGEN Sesame |
The real-time PCR test detects DNA of sesame (Sesamum indicum) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3608 |
| SureFood® ALLERGEN Walnut |
The SureFood® ALLERGEN Walnut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific walnut (Juglans regia and Juglans nigra) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination … Read more |
100 reactions | S3607 |
| SureFood® ALLERGEN Pistachio |
The SureFood® ALLERGEN Pistachio is a real-time PCR for the direct, qualitative and / or quantitative detection of specific pistachio (Pistacia vera) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination the use of … Read more |
100 reactions | S3614 |
| SureFood® ALLERGEN Pecan |
The SureFood® ALLERGEN Pecan is a real-time PCR for the direct, qualitative and / or quantitative detection of specific pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination the use of … Read more |
100 reactions | S3618 |
| SureFood® ALLERGEN Peanut |
The real-time PCR test detects DNA of peanut (Arachis hypogaea) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3603 |
| SureFood® ALLERGEN Macadamia |
The real-time PCR test detects DNA of macadamia (Macadamia ternifolia) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the … Read more |
100 reactions | S3616 |
| SureFood® ALLERGEN Hazelnut |
The real-time PCR test detects DNA of hazelnut (Corylus avellana, Corylus maxima) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative … Read more |
100 reactions | S3602 |
| SureFood® ALLERGEN Cashew |
The real-time PCR test detects DNA of cashew (Anacardium occidentale) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the … Read more |
100 reactions | S3615 |
| SureFood® ALLERGEN Brazil Nut |
The SureFood® ALLERGEN Brazil Nut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific brazil nut (Bertholletia excelsa) DNA sequences according to directive (EC) 1169/2011. For the quantitative determination … Read more |
100 reactions | S3617 |
| SureFood® ALLERGEN Almond |
The real-time PCR test detects DNA of almond (Prunus dulcis) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of … Read more |
100 reactions | S3604 |
| SureFood® ALLERGEN Soya |
The real-time PCR test detects soya DNA (Glycine max) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC). For the quantitative determination the use of the … Read more |
100 reactions | S3601 |
| SureFood® ALLERGEN Gluten |
The SureFood® ALLERGEN Gluten is a real-time PCR for the direct, qualitative and / or quantitative detection of specific gluten-containing cereals (wheat such as spelt and khorasan wheat, rye, barley, oats) DNA sequences according to directive (EC) … Read more |
100 reactions | S3606 |
| SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC |
The SureFood® ALLERGEN 4plex Soya / Celery / Mustard + IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific celery (Apium graveolens), mustard (Brassica carinata, Brassica juncea, Brassica nigra, … Read more |
100 reactions | S3401 |
| SureFood® QUANTARD Allergen 40 |
SureFood® QUANTARD Allergen 40 is a laboratory reference material developed for quantification in the range of 1 – 400 mg allergenic substance / kg food. It is verified in combination with the DNA extraction method SureFood® PREP Advanced … Read more |
1x 2 g | S3301 |
| SureFood® ALLERGEN 4plex Peanut / Hazelnut / Walnut + IAC |
The SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific Peanut (Arachis hypogaea), Hazelnut (Corylus maxima) and Walnut (Juglans regia, Juglans nigra) … Read more |
100 reactions | S3402 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Read more |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Read more |
96 preparations | F1060 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Read more |
Up to 48 samples | ZMAL48 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Read more |
96 preparations | F1060 |
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