Real-time PCR

Molecular diagnostics in food analysis

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Real-time PCR

PCR (short for polymerase chain reaction) is an important analysis method in food analysis as well as in medical diagnostics.

A real-time PCR test duplicates and analyzes specific DNA sequences. In food analysis, PCR tests are used in many applications, e.g. for the detection of pathogenic microorganisms, for allergen identification, for the detection of genetically modified organisms (GMO) or for the identification of animal species. PCR tests offer many advantages: They are very specific, sensitive, robust, rapid, reliable and moreover, the tests can be automated.

  • Safe: specific and validated method
  • Reliable: quality-controlled test kits
  • User-friendly: easy-to-use, universal sample preparation
  • Effective: capable to multiplex and quantify
  • Versatile: many application areas
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Real-time PCR explained

The first step of all analysis is representative sampling and homogenization. After lysis of the organic cells of food tissue, the DNA will be prepared using different dedicated DNA or RNA preparation kits either for bacteria or viruses.

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Your way to automation

Discover the new walk-away solution: Semi-automated nucleic acid preparation for all food and feed samples with the TANBead Maelstrom™ 8 Autostage and the extraction kit SureFast® Mag PREP Food.

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The multiplex real-time PCR thermocycler

RIDA®CYCLER is a compact and easy-to-use real-time PCR cycler that allows fast and flexible analysis of your food & feed samples. The user-friendly software enables a fast analysis start of one or more tests simultaneously.

SureFood® PREP Basic and SureFood® Prep Advanced kits are used for solid food and feed from plant or meat origin. For beverages specific DNA preparation kits will be used depending on the matrix, whereas the new QuickGEN line allows a straight forward easy handling.

The modular system of DNA preparation and DNA detection can be used independently. Amplification of the target DNA requires in addition to the relevant kits the use of a suitable thermocycler with two (FAM/HEX) up to 4 (FAM/HEX/ROX/Cy5) detection channels.

Application areas for PCR in food testing

Real-time PCR Product Overview

Food allergies can cause severe immunological reactions. In order to protect customers, food products have to be tested for allergenic substances. We offer a wide range of PCR kits for the detection of allergens such as soya, hazelnut, peanut, gluten, almond, celery, sesame, mustard, fish or crustaceans. We also offer multiplex assays for the simultaneous detection of multiple allergens (peanut/hazelnut/walnut and celery/soya/mustard).

Species specification is of great interest: meat products can be falsified due to incorrect declaration (see horse meat scandal). Another application is the guarantee of religious dietary rules (halal, kosher). Our SureFood® Animal ID product line allows for sensitive testing for pork, beef, horse, donkey, chicken, turkey and other species. With the SureFood® Fish ID product line, we offer a wide range of PCR kits for the identification of fish species.

When producing beverages, the detection of beverage spoilers is crucial. With the GEN-IAL® product line, we offer a wide range of screening kits for the detection of spoilage organisms in beer and wine production, e.g. Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis or Oenococcus oeni. The test kits can be used for in-process control as well as for final product control.

In recent years, the worldwide production of genetically modified food has steadily increased. Since food products, which contain genetically modified organisms (GMO), must be labelled, the demand for sensitive test methods is increasing. Our SureFood® GMO product line allows for qualitative and quantitative detection of GMO – such as genetically modified maize, LibertyLink Rice or Roundup Ready Soya.

Detection of microbiological contamination is one of the main fields of application of PCR in food analysis. Our product line SureFast® detects a wide range of pathogenic bacteria (e.g. Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), viruses (e.g. Norovirus, Hepatitis and Influenza) as well as spoilage yeast and bacteria.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

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Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors …

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96 preparations F1060
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ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Read more
Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors …

Read more
96 preparations F1060
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ProductDescriptionNo. of tests/amountArt. No.
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Read more
Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors …

Read more
96 preparations F1060
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ProductDescriptionNo. of tests/amountArt. No.
SureFast® Enterobacteriaceae 4plex

The SureFast® Enterobacteriaceae 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacteriaceae, Cronobacter spp. and Salmonella spp..

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100 reactions F5180
SureFast® Fecal Screen 4plex

The SureFast® Fecal Screen 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacteriaceae, Enterococci, Escherichia coli in water.

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100 reactions F5504
QuickGEN PCR Kit Yeast universal -low-

Qualitative detection of yeast contaminations in beverages. For exapmple: Saccharomyces spp. Dekkera spp. Zygosaccharomyces spp. Rhodotorula spp. Torulaspora spp. Kluyveromyces spp. Debaromyces spp. Candida spp. Hanseniaspora spp. Pichia spp

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48 reactions: 48 x Dye Strips (freeze-dried, incl. IC-DNA) Q982
GEN-IAL® QuickGEN P1 and Dekkera spp. Screening low MG

Qualitative detection of bacterial and Dekkera spp. contaminations in filtrated beer, beer mixes and yeast containing samples.

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48 reactions Q094
SureFast® Listeria 3plex ONE

The SureFast® Listeria 3plex ONE can be applied for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food. The method consists of the following three steps: 1) Cultural enrichment 2) …

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100 reactions F5217
QuickGEN PCR Kit Fusarium spp.

Detection of Fusarium spp. in beverages, cereals etc. by real-time PCR.

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48 reactions Q961
QuickGEN Pentaplex PCR Kit – Beer spoilage bacteria and yeast screening

Qualitative detection of bacterial, S. cerevisiae var. diastaticus and Dekkera spp. contaminations in filtered beer, beer mixes and yeast containing samples.

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48 reactions Q061
SureFast® Campylobacter 4plex

The SureFast® Campylobacter 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Campylobacter jejuni, Campylobacter lari and Campylobacter coli.

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100 reactions F5170
SureFast® Mag PREP Pathogens

The kit is intended to be used for the isolation of DNA and RNA viruses nucleic acids from food and feed (e.g. wash up fluids from fruits, salads etc.), filters from water samples and swabs (e.g. from work surfaces etc.) as well as bacterial DNA from …

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96 preparations F1062
SureFast® STEC 4plex ONE

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli …

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100 reactions F5265
SureFast® MRSA 4plex

The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of …

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100 reactions F7117
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Read more
Up to 48 samples ZMAL48
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors …

Read more
96 preparations F1060
GEN-IAL® QuickGEN PCR Kit Alicyclobacillus Differentiation high

Qualitative detection and differentiation of Alicyclobacillus spp., Alicyclobacillus acidocaldarius and Alicyclobacillus acidoterrestris .

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48 reactions Q721
SureFast® Yersinia 3plex

The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica.

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100 reactions F5132
GEN-IAL® Dekkera bruxellensis Standard DNA

Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR.

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1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried) Q360
GEN-IAL® QuickGEN PCR Kit S. cerevisiae var. diastaticus white

Detection of S. cerevisiae var. diastaticus in beverages.

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48 reactions Q183
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions Q563
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions (tubes incl. Lyticase and IC-DNA) Q562
GEN-IAL® QuickGEN Yeast Dekkera spp.

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus.

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50 reactions Q555
GEN-IAL® QuickGEN Yeast Dekkera spp. white

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q553
GEN-IAL® QuickGEN Yeast Dekkera spp. low

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus

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48 reactions Q552
GEN-IAL® QuickGEN Yeast Dekkera spp. high

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q551
GEN-IAL® QuickGEN Yeast differentiation white

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples Q543
GEN-IAL® QuickGEN Yeast differentiation low

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples (tubes contain lyticase). Q542
GEN-IAL® QuickGEN Wild yeast 2 low

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia …

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48 reactions Q532
GEN-IAL® QuickGEN Wild yeast 1

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila …

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50 reactions Q525
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/HEX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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50 reactions Q395
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/ROX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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50 reactions Q385
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative white

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions Q373
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative low

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

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48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). Q372
GEN-IAL® QuickGEN Wine Screening without yeast low MG

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q334
GEN-IAL® QuickGEN Wine Screening without yeast white

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q333
GEN-IAL® QuickGEN Wine Screening without yeast low

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

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48 reactions Q332
GEN-IAL® QuickGEN Wine Screening low MG

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

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48 reactions Q324
GEN-IAL® QuickGEN Wine Screening white

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

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48 reactions Q323
GEN-IAL® QuickGEN Wine Screening low

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

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48 reactions Q322
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high

Detection of Saccharomyces diastaticus in beverages by real-time PCR.

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48 reactions Q181
GEN-IAL® QuickGEN Yeast Bottom fermented white

Detection of bottom-fermented yeast in beverages by real-time PCR.

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48 reactions Q163
GEN-IAL® QuickGEN Yeast Bottom fermented low

Detection of bottom-fermented yeast in beverages (e.g. beer, wine, grape must) by real-time PCR.

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48 reactions Q162
GEN-IAL® QuickGEN® Yeast Top fermented white

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q153
GEN-IAL® QuickGEN® Yeast Top fermented low

Real-time PCR-Kit for the detection of top-fermented yeast.

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48 reactions Q152
GEN-IAL® QuickGEN Beer differentiation white

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q083
GEN-IAL® QuickGEN Beer differentiation low

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. …

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12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. Q082
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation white

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

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96 PCR reactions (24 samples)
12 stripes (tubes 2, 3, 5 and 6 contain lyticase)
Q073
GEN-IAL® QuickGEN Beer yeast and bacteria differentiation low

Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild …

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96 PCR reactions (24 samples)
12 stripes
Q072
GEN-IAL® QuickGEN P1 Screening and Hop resistance

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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50 reactions Q055
GEN-IAL® QuickGEN P1 Screening and Hop resistance low MG

Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks.

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48 reactions Q054
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